MultiFit: A web server for fitting multiple protein structures into their electron microscopy density map

Elina Tjioe, Keren Lasker, Ben Webb, Haim J. Wolfson*, Andrej Sali

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Advances in electron microscopy (EM) allow for structure determination of large biological assemblies at increasingly higher resolutions. A key step in this process is fitting multiple component structures into an EM-derived density map of their assembly. Here, we describe a web server for this task. The server takes as input a set of protein structures in the PDB format and an EM density map in the MRC format. The output is an ensemble of models ranked by their quality of fit to the density map. The models can be viewed online or downloaded from the website. The service is available at; http://salilab.org/multifit/ and http://bioinfo3d.cs.tau.ac.il/.

Original languageEnglish
Pages (from-to)W167-W170
JournalNucleic Acids Research
Volume39
Issue numberSUPPL. 2
DOIs
StatePublished - 1 Jul 2011

Funding

FundersFunder number
Hermann Minkowski-MINERVA Center for Geometry
Sandler Family Supporting Foundation
National Institutes of HealthU54 RR022220, R01 GM083960, R01 GM54762
National Eye InstitutePN2EY016525
Intel Corporation
International Business Machines Corporation
Israel Science Foundation1403/09
Tel Aviv University

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