TY - GEN
T1 - Multicomponent analysis of human blood using fiber optic evanescent wave spectroscopy
AU - Simhi, Ronit
AU - Bunimovich, D.
AU - Sela, B.
AU - Katzir, Abraham
PY - 1995
Y1 - 1995
N2 - Blood analysis has been carried out routinely for decades. Normally, blood samples are taken from patients and sent to the laboratory for processing. Novel analytical methods are facilitated by fiberoptic sensors that are inserted into the human body or in contact with it. In the present work a spectral analysis of human blood serum was undertaken by fiberoptic evanescent wave spectroscopy (FEWS) using a Fourier transform infrared spectrometer (FTIR). A special cell based on IR transmitting non-toxic silver halide fibers was designed. Further improvements in the analysis were obtained by adopting some multivariate calibration techniques that have already been used in clinical chemistry. The partial least-square (PLS) algorithm was used to calculate the concentrations of urea, uric acid, calcium, cholesterol and total protein in human blood serum. Good agreement between our results and the ordinary chemical and enzymatic methods was reached. The average errors obtained were 28 mg/dl for cholesterol (about 16% of the average value), 9 mg/dl for urea (about 29% of the average value), 0.38 g/L for total protein (about 6% of the average value), 1.5 mg/dl for uric acid (about 29% of the average value) and 0.28 mg/dl for calcium (about 3% of the average value).
AB - Blood analysis has been carried out routinely for decades. Normally, blood samples are taken from patients and sent to the laboratory for processing. Novel analytical methods are facilitated by fiberoptic sensors that are inserted into the human body or in contact with it. In the present work a spectral analysis of human blood serum was undertaken by fiberoptic evanescent wave spectroscopy (FEWS) using a Fourier transform infrared spectrometer (FTIR). A special cell based on IR transmitting non-toxic silver halide fibers was designed. Further improvements in the analysis were obtained by adopting some multivariate calibration techniques that have already been used in clinical chemistry. The partial least-square (PLS) algorithm was used to calculate the concentrations of urea, uric acid, calcium, cholesterol and total protein in human blood serum. Good agreement between our results and the ordinary chemical and enzymatic methods was reached. The average errors obtained were 28 mg/dl for cholesterol (about 16% of the average value), 9 mg/dl for urea (about 29% of the average value), 0.38 g/L for total protein (about 6% of the average value), 1.5 mg/dl for uric acid (about 29% of the average value) and 0.28 mg/dl for calcium (about 3% of the average value).
UR - http://www.scopus.com/inward/record.url?scp=0029224673&partnerID=8YFLogxK
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AN - SCOPUS:0029224673
SN - 0819416649
SN - 9780819416643
T3 - Proceedings of SPIE - The International Society for Optical Engineering
SP - 166
EP - 172
BT - Proceedings of SPIE - The International Society for Optical Engineering
A2 - Scheggi, Annamaria V.
A2 - Baldini, Francesco
A2 - Coulet, Pierre R.
A2 - Wolfbeis, Otto S.
T2 - Medical Sensors II and Fiber Optic Sensors
Y2 - 9 September 1994 through 9 September 1994
ER -