Multicomponent analysis of human blood using fiber optic evanescent wave spectroscopy

Ronit Simhi, D. Bunimovich, B. Sela, Abraham Katzir

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Blood analysis has been carried out routinely for decades. Normally, blood samples are taken from patients and sent to the laboratory for processing. Novel analytical methods are facilitated by fiberoptic sensors that are inserted into the human body or in contact with it. In the present work a spectral analysis of human blood serum was undertaken by fiberoptic evanescent wave spectroscopy (FEWS) using a Fourier transform infrared spectrometer (FTIR). A special cell based on IR transmitting non-toxic silver halide fibers was designed. Further improvements in the analysis were obtained by adopting some multivariate calibration techniques that have already been used in clinical chemistry. The partial least-square (PLS) algorithm was used to calculate the concentrations of urea, uric acid, calcium, cholesterol and total protein in human blood serum. Good agreement between our results and the ordinary chemical and enzymatic methods was reached. The average errors obtained were 28 mg/dl for cholesterol (about 16% of the average value), 9 mg/dl for urea (about 29% of the average value), 0.38 g/L for total protein (about 6% of the average value), 1.5 mg/dl for uric acid (about 29% of the average value) and 0.28 mg/dl for calcium (about 3% of the average value).

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsAnnamaria V. Scheggi, Francesco Baldini, Pierre R. Coulet, Otto S. Wolfbeis
Pages166-172
Number of pages7
StatePublished - 1995
EventMedical Sensors II and Fiber Optic Sensors - Lille, Fr
Duration: 9 Sep 19949 Sep 1994

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2331
ISSN (Print)0277-786X

Conference

ConferenceMedical Sensors II and Fiber Optic Sensors
CityLille, Fr
Period9/09/949/09/94

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