TY - JOUR
T1 - Multi-parametric characterization of drug effects on cells [version 2; peer review
T2 - 2 approved]
AU - Paran, Yael
AU - Liron, Yuvalal
AU - Batsir, Sarit
AU - Mabjeesh, Nicola
AU - Geiger, Benjamin
AU - Kam, Zvi
N1 - Publisher Copyright:
© 2021. Paran Y et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
PY - 2021
Y1 - 2021
N2 - We present here a novel multi-parametric approach for the characterization of multiple cellular features, using images acquiredby high-throughput and high-definition light microscopy. We specifically used this approach for deep and unbiased analysis of the effects of a drug library on five cultured cell lines. The presented method enables the acquisition and analysis of millions of images, of treated and control cells, followed by an automated identification of drugs inducing strong responses, evaluating the median effect concentrations and those cellular properties that are most highly affected by the drug. The tools described here provide standardized quantification of multiple attributes for systems level dissection of complex functions in normal and diseased cells, using multiple perturbations. Such analysis of cells, derived from pathological samples, may help in the diagnosis and follow-up of treatment in patients.
AB - We present here a novel multi-parametric approach for the characterization of multiple cellular features, using images acquiredby high-throughput and high-definition light microscopy. We specifically used this approach for deep and unbiased analysis of the effects of a drug library on five cultured cell lines. The presented method enables the acquisition and analysis of millions of images, of treated and control cells, followed by an automated identification of drugs inducing strong responses, evaluating the median effect concentrations and those cellular properties that are most highly affected by the drug. The tools described here provide standardized quantification of multiple attributes for systems level dissection of complex functions in normal and diseased cells, using multiple perturbations. Such analysis of cells, derived from pathological samples, may help in the diagnosis and follow-up of treatment in patients.
KW - Drug effect screening
KW - Microscope imaging
KW - Multiparameter scoring
UR - http://www.scopus.com/inward/record.url?scp=85117317562&partnerID=8YFLogxK
U2 - 10.12688/F1000RESEARCH.26254.2
DO - 10.12688/F1000RESEARCH.26254.2
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:85117317562
SN - 2046-1402
VL - 9
SP - 1
EP - 16
JO - F1000Research
JF - F1000Research
ER -