Mucosal IL-10 and IL-10 receptor expression patterns in paediatric patients with ulcerative colitis

Talya Wittmann Dayagi, Lael Werner, Marina Pinsker, Naomi Salamon, Iris Barschak, Batia Weiss, Dror S. Shouval*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Interleukin-10 (IL-10) is a key anti-inflammatory cytokine. We aimed to assess IL-10 and IL-10 receptor (IL-10R) expression in the gut, and determine whether these patterns are altered in patients with ulcerative colitis (UC). Formalin-fixed paraffin-embedded rectal and transverse colon sections were collected from three groups of patients: (a) control subjects with normal colonoscopy and without history of inflammatory bowel disease; (b) UC patients with extensive colitis or pancolitis (E3/E4 phenotype); and (c) UC patients with limited distal disease (E1/E2 phenotype; n = 8-10 subjects per group). Immunohistochemistry (IHC) was performed to assess expression patterns of IL-10, IL-10R1 and IL-10R2, and was correlated with clinical, endoscopic and histologic severity indices among patients. A trend towards increased IL-10 expression was noted in rectal biopsies of patients with active UC, compared with controls. Moreover, IL-10 levels were significantly increased in transverse colon biopsies of patients with extensive/pancolitis, compared with control subjects and patients with limited distal disease. Rectal IL-10R1 and IL-10R2 levels were comparable between control subject and patients with active UC. However, transverse colon IL-10R1 levels were significantly higher in patients with E3/E4 colitis, compared with controls. Finally, we found no correlation between clinical, endoscopic and histologic severity of inflammation among UC patients and IL-10, IL-10R1 or IL-10R2 expression in rectal sections. Mucosal expression patterns of IL-10 and IL-10R, evaluated by IHC, were overall similar between control subjects and patients with active UC. Given IL-10’s anti-inflammatory properties, additional studies are required to determine whether signalling through the IL-10R is altered among these patients.

Original languageEnglish
Pages (from-to)4-10
Number of pages7
JournalInternational Journal of Experimental Pathology
Volume102
Issue number1
DOIs
StatePublished - Feb 2021

Funding

FundersFunder number
Israel Science Foundation1897/16

    Keywords

    • IL-10
    • IL-10R
    • inflammatory bowel diseases
    • mucosal homeostasis
    • pathology
    • ulcerative colitis

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