mRNA m6A detection

Sharon Moshitch-Moshkovitz; Michal Sevilla-Sharon; Reut Ashwal-Fluss; Efrat Glick-Saar; Gideon Rechavi; Dan Dominissini

doi:10.1038/s43586-024-00365-9

mRNA m⁶A detection

Sharon Moshitch-Moshkovitz^*, Michal Sevilla-Sharon, Reut Ashwal-Fluss, Efrat Glick-Saar, Gideon Rechavi, Dan Dominissini

^*Corresponding author for this work

Sheba Medical Center at Tel Hashomer

Research output: Contribution to journal › Article › peer-review

Abstract

N⁶-methyladenosine (m⁶A) is the most prevalent internal mRNA modification. Recent research has highlighted its role as a key regulator of gene expression, influencing cellular processes and determining cell fate. Advances in techniques for global mapping of m⁶A, the discovery of m⁶A demethylases that enhance its dynamic properties and the identification of reader proteins that interact with m⁶A have substantially propelled this field forward. This Primer outlines the available tools for detecting and mapping m⁶A, discusses the strengths and limitations of each method and offers guidance on selecting the most suitable approach. Identifying and detecting m⁶A lays the groundwork for functional studies that address important biological and medical questions.

Original language	English
Article number	87
Journal	Nature Reviews Methods Primers
Volume	4
Issue number	1
DOIs	https://doi.org/10.1038/s43586-024-00365-9
State	Published - Dec 2024

Funding

Funders	Funder number
German–Israeli Project Cooperation
DIP
Israel Innovation Authority
National Headquarter ‘Digital Israel’
Bundesministerium für Bildung und Forschung
Ministry of Health, State of Israel
Flight Attendant Medical Research Institute
Tel Aviv University
European Research Council
Varda and Boaz Dotan Research Center in Hemato-Oncology
Israel Science Foundation	2494/18, 2625/17
Human Frontier Science Program	CDA 00048/2018
Horizon 2020 Framework Programme	743168

Access to Document

10.1038/s43586-024-00365-9

Cite this

@article{f8e28ad7af944820b0ca9b1d203eb0df,

title = "mRNA m6A detection",

abstract = "N6-methyladenosine (m6A) is the most prevalent internal mRNA modification. Recent research has highlighted its role as a key regulator of gene expression, influencing cellular processes and determining cell fate. Advances in techniques for global mapping of m6A, the discovery of m6A demethylases that enhance its dynamic properties and the identification of reader proteins that interact with m6A have substantially propelled this field forward. This Primer outlines the available tools for detecting and mapping m6A, discusses the strengths and limitations of each method and offers guidance on selecting the most suitable approach. Identifying and detecting m6A lays the groundwork for functional studies that address important biological and medical questions.",

author = "Sharon Moshitch-Moshkovitz and Michal Sevilla-Sharon and Reut Ashwal-Fluss and Efrat Glick-Saar and Gideon Rechavi and Dan Dominissini",

note = "Publisher Copyright: {\textcopyright} Springer Nature Limited 2024.",

year = "2024",

month = dec,

doi = "10.1038/s43586-024-00365-9",

language = "אנגלית",

volume = "4",

journal = "Nature Reviews Methods Primers",

issn = "2662-8449",

publisher = "Springer Nature",

number = "1",

}

TY - JOUR

T1 - mRNA m6A detection

AU - Moshitch-Moshkovitz, Sharon

AU - Sevilla-Sharon, Michal

AU - Ashwal-Fluss, Reut

AU - Glick-Saar, Efrat

AU - Rechavi, Gideon

AU - Dominissini, Dan

PY - 2024/12

Y1 - 2024/12

N2 - N6-methyladenosine (m6A) is the most prevalent internal mRNA modification. Recent research has highlighted its role as a key regulator of gene expression, influencing cellular processes and determining cell fate. Advances in techniques for global mapping of m6A, the discovery of m6A demethylases that enhance its dynamic properties and the identification of reader proteins that interact with m6A have substantially propelled this field forward. This Primer outlines the available tools for detecting and mapping m6A, discusses the strengths and limitations of each method and offers guidance on selecting the most suitable approach. Identifying and detecting m6A lays the groundwork for functional studies that address important biological and medical questions.

AB - N6-methyladenosine (m6A) is the most prevalent internal mRNA modification. Recent research has highlighted its role as a key regulator of gene expression, influencing cellular processes and determining cell fate. Advances in techniques for global mapping of m6A, the discovery of m6A demethylases that enhance its dynamic properties and the identification of reader proteins that interact with m6A have substantially propelled this field forward. This Primer outlines the available tools for detecting and mapping m6A, discusses the strengths and limitations of each method and offers guidance on selecting the most suitable approach. Identifying and detecting m6A lays the groundwork for functional studies that address important biological and medical questions.

UR - http://www.scopus.com/inward/record.url?scp=85211780475&partnerID=8YFLogxK

U2 - 10.1038/s43586-024-00365-9

DO - 10.1038/s43586-024-00365-9

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:85211780475

SN - 2662-8449

VL - 4

JO - Nature Reviews Methods Primers

JF - Nature Reviews Methods Primers

IS - 1

M1 - 87

ER -

mRNA m⁶A detection

Abstract

Funding

Access to Document

Other files and links

Fingerprint

Cite this