Mouse models of spontaneous liver and lung metastasis for colorectal cancer

Jöran Lücke*, Baris Mercanoglu, Tao Zhang, Dimitra E. Zazara, Ehud Zigmond, Philipp Seeger, Oliver Mann, Jakob R. Izbicki, Thilo Hackert, Samuel Huber, Anastasios D. Giannou*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


To investigate underlying mechanisms for cancer metastasis and promising therapies in animal models, spontaneous metastasis models can be used to recreate metastasis development. Here, we present three mouse models of spontaneous lung and/or liver metastasis induction. We describe steps for cancer cell preparation, mouse analgesia, and three injection techniques (subcutaneous, intracecal, and intramucosal). We then detail procedures for evaluating metastasis. Most of these models generate metastasis in a time span of 4 weeks in the majority of injected mice. For complete details on the use and execution of this protocol, please refer to Giannou et al.1

Original languageEnglish
Article number102811
JournalSTAR Protocols
Issue number1
StatePublished - 15 Mar 2024


FundersFunder number
Hamburger Krebsgesellschaft Stiftung
Erich und Gertrud Roggenbuck-Stiftung
Deutsche ForschungsgemeinschaftSFB841
Jung-Stiftung für Wissenschaft und Forschung
Deutsche Krebshilfe70114853


    • Cancer
    • Immunology
    • Model Organisms


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