Morphine Inhibits Calcium Influx and the Response to Acetylcholine in Xenopus Oocytes

D. Lipinsky; Y. Oron; Y. Sarne

doi:10.1111/j.1471-4159.1993.tb05851.x

Morphine Inhibits Calcium Influx and the Response to Acetylcholine in Xenopus Oocytes

D. Lipinsky, Y. Oron, Y. Sarne^*

^*Corresponding author for this work

Physiology Pharmacology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Abstract: Incubation of intact Xenopus oocytes with the opioid radioligand [³H]diprenorphine (0.5 nM) resulted in specific binding of 1.7 ± 0.3 fmol per oocyte. Morphine (10 μM) inhibited the uptake of ⁴⁵Ca²⁺ into the oocyte by 66 ± 9%. The opioid antagonist naltrexone partially blocked this effect of morphine. Preincubation of oocytes with morphine (10 μM, 2 min) partially inhibited the fast and slow responses of the oocyte to acetylcholine by 26 and 52%, respectively. We conclude that native Xenopus oocytes possess opioid receptors that may modulate the muscarinic response by limiting calcium influx into the cell.

Original language	English
Pages (from-to)	299-304
Number of pages	6
Journal	Journal of Neurochemistry
Volume	60
Issue number	1
DOIs	https://doi.org/10.1111/j.1471-4159.1993.tb05851.x
State	Published - Jan 1993

Keywords

Acetylcholine
Ca fluxes
Morphine
Muscarinic response
Opioid receptor
Xenopus oocyte

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10.1111/j.1471-4159.1993.tb05851.x

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abstract = "Abstract: Incubation of intact Xenopus oocytes with the opioid radioligand [3H]diprenorphine (0.5 nM) resulted in specific binding of 1.7 ± 0.3 fmol per oocyte. Morphine (10 μM) inhibited the uptake of 45Ca2+ into the oocyte by 66 ± 9%. The opioid antagonist naltrexone partially blocked this effect of morphine. Preincubation of oocytes with morphine (10 μM, 2 min) partially inhibited the fast and slow responses of the oocyte to acetylcholine by 26 and 52%, respectively. We conclude that native Xenopus oocytes possess opioid receptors that may modulate the muscarinic response by limiting calcium influx into the cell.",

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N2 - Abstract: Incubation of intact Xenopus oocytes with the opioid radioligand [3H]diprenorphine (0.5 nM) resulted in specific binding of 1.7 ± 0.3 fmol per oocyte. Morphine (10 μM) inhibited the uptake of 45Ca2+ into the oocyte by 66 ± 9%. The opioid antagonist naltrexone partially blocked this effect of morphine. Preincubation of oocytes with morphine (10 μM, 2 min) partially inhibited the fast and slow responses of the oocyte to acetylcholine by 26 and 52%, respectively. We conclude that native Xenopus oocytes possess opioid receptors that may modulate the muscarinic response by limiting calcium influx into the cell.

AB - Abstract: Incubation of intact Xenopus oocytes with the opioid radioligand [3H]diprenorphine (0.5 nM) resulted in specific binding of 1.7 ± 0.3 fmol per oocyte. Morphine (10 μM) inhibited the uptake of 45Ca2+ into the oocyte by 66 ± 9%. The opioid antagonist naltrexone partially blocked this effect of morphine. Preincubation of oocytes with morphine (10 μM, 2 min) partially inhibited the fast and slow responses of the oocyte to acetylcholine by 26 and 52%, respectively. We conclude that native Xenopus oocytes possess opioid receptors that may modulate the muscarinic response by limiting calcium influx into the cell.

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KW - Ca fluxes

KW - Morphine

KW - Muscarinic response

KW - Opioid receptor

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Morphine Inhibits Calcium Influx and the Response to Acetylcholine in Xenopus Oocytes

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