Morphine Inhibits Calcium Influx and the Response to Acetylcholine in Xenopus Oocytes

D. Lipinsky, Y. Oron, Y. Sarne

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: Incubation of intact Xenopus oocytes with the opioid radioligand [3H]diprenorphine (0.5 nM) resulted in specific binding of 1.7 ± 0.3 fmol per oocyte. Morphine (10 μM) inhibited the uptake of 45Ca2+ into the oocyte by 66 ± 9%. The opioid antagonist naltrexone partially blocked this effect of morphine. Preincubation of oocytes with morphine (10 μM, 2 min) partially inhibited the fast and slow responses of the oocyte to acetylcholine by 26 and 52%, respectively. We conclude that native Xenopus oocytes possess opioid receptors that may modulate the muscarinic response by limiting calcium influx into the cell.

Original languageEnglish
Pages (from-to)299-304
Number of pages6
JournalJournal of Neurochemistry
Volume60
Issue number1
DOIs
StatePublished - Jan 1993

Keywords

  • Acetylcholine
  • Ca fluxes
  • Morphine
  • Muscarinic response
  • Opioid receptor
  • Xenopus oocyte

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