Monofunctional Substrates of Polynucleotide Phosphorylase: The Monoaddition of 2′(3′)‐O‐Isovaleryl‐nucleoside Diphosphate to an Initiator Oligonucleotide

Gabriel Kaufmann, Matityahu Fridkin, Aliza Zutra, Uriel Z. Littauer

Research output: Contribution to journalArticlepeer-review

Abstract

Polynucleotide phosphorylase from Escherichia coli cells catalyzes the transfer of a single nucleotidyl residue from each of the 2′(3′)‐O‐isovalerylesters of ADP, GDP, CDP and UDP to the initiator oligonucleotide, A‐A‐A. The products of these reactions are identified as the 2′(3′)‐O‐isovaleryl esters of the corresponding tetranucleotides A‐A‐A‐A, A‐A‐A‐G, A‐A‐A‐C and A‐A‐A‐U. The 2′(3′)‐O‐isovaleryl residue can be removed from the product by treatment with aqueous methanolic ammonia under conditions that do not significantly damage the oligonucleotide chains. The monoaddition of 2′(3′)‐O‐acyl esters of nucleoside diphosphates to an oligonucleotide acceptor is proposed as a method for the stepwise synthesis of oligoribonucleotides of defined sequence.

Original languageEnglish
Pages (from-to)4-11
Number of pages8
JournalEuropean Journal of Biochemistry
Volume24
Issue number1
DOIs
StatePublished - Dec 1971
Externally publishedYes

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