Monkeypox infection elicits strong antibody and B cell response against A35R and H3L antigens

Ron Yefet, Nadav Friedel, Hadas Tamir, Ksenia Polonsky, Michael Mor, Lilach Cherry-Mimran, Eyal Taleb, David Hagin, Eli Sprecher, Tomer Israely, Natalia T. Freund*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Monkeypox virus (MPXV) resides in two forms; mature and enveloped, and depending on it, distinct proteins are displayed on the viral surface. Here, we expressed two MPXV antigens from the mature, and one from the enveloped form, and tested their reactivity to sera of 11 MPXV recoverees while comparing to sera from recently and past vaccinated individuals. 8 out of 11 recoverees exhibited detectable neutralization levels against Vaccinia Lister. Sera from all recoverees bound strongly to A35R and H3L antigens. Moreover, the responses to A35R were significantly higher within the recoverees compared to both recently and past vaccinated donors. Lastly, A35R- and H3L-specific IgG+ B cells ranging from 0.03-0.46% and 0.11–0.36%, respectively, were detected in all recoverees (A35R), and in 9 out of 11 recoverees (H3L). Therefore, A35R and H3L represent MPXV immune targets and could be used in a heat-inactivated serological ELISA for the identification of recent MPXV infection.

Original languageEnglish
Article number105957
Issue number2
StatePublished - 17 Feb 2023


FundersFunder number
Edmond J. Safra Center for Bioinformatics
Tel Aviv University
Israel Institute for Biological Research


    • Immunological methods
    • Immunology
    • Virology


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