TY - JOUR
T1 - Monkeypox infection elicits strong antibody and B cell response against A35R and H3L antigens
AU - Yefet, Ron
AU - Friedel, Nadav
AU - Tamir, Hadas
AU - Polonsky, Ksenia
AU - Mor, Michael
AU - Cherry-Mimran, Lilach
AU - Taleb, Eyal
AU - Hagin, David
AU - Sprecher, Eli
AU - Israely, Tomer
AU - Freund, Natalia T.
N1 - Publisher Copyright:
© 2023 The Author(s)
PY - 2023/2/17
Y1 - 2023/2/17
N2 - Monkeypox virus (MPXV) resides in two forms; mature and enveloped, and depending on it, distinct proteins are displayed on the viral surface. Here, we expressed two MPXV antigens from the mature, and one from the enveloped form, and tested their reactivity to sera of 11 MPXV recoverees while comparing to sera from recently and past vaccinated individuals. 8 out of 11 recoverees exhibited detectable neutralization levels against Vaccinia Lister. Sera from all recoverees bound strongly to A35R and H3L antigens. Moreover, the responses to A35R were significantly higher within the recoverees compared to both recently and past vaccinated donors. Lastly, A35R- and H3L-specific IgG+ B cells ranging from 0.03-0.46% and 0.11–0.36%, respectively, were detected in all recoverees (A35R), and in 9 out of 11 recoverees (H3L). Therefore, A35R and H3L represent MPXV immune targets and could be used in a heat-inactivated serological ELISA for the identification of recent MPXV infection.
AB - Monkeypox virus (MPXV) resides in two forms; mature and enveloped, and depending on it, distinct proteins are displayed on the viral surface. Here, we expressed two MPXV antigens from the mature, and one from the enveloped form, and tested their reactivity to sera of 11 MPXV recoverees while comparing to sera from recently and past vaccinated individuals. 8 out of 11 recoverees exhibited detectable neutralization levels against Vaccinia Lister. Sera from all recoverees bound strongly to A35R and H3L antigens. Moreover, the responses to A35R were significantly higher within the recoverees compared to both recently and past vaccinated donors. Lastly, A35R- and H3L-specific IgG+ B cells ranging from 0.03-0.46% and 0.11–0.36%, respectively, were detected in all recoverees (A35R), and in 9 out of 11 recoverees (H3L). Therefore, A35R and H3L represent MPXV immune targets and could be used in a heat-inactivated serological ELISA for the identification of recent MPXV infection.
KW - Immunological methods
KW - Immunology
KW - Virology
UR - http://www.scopus.com/inward/record.url?scp=85146845692&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2023.105957
DO - 10.1016/j.isci.2023.105957
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C2 - 36687315
AN - SCOPUS:85146845692
SN - 2589-0042
VL - 26
JO - iScience
JF - iScience
IS - 2
M1 - 105957
ER -