Monitoring the transport and phosphorylation of 2-deoxy-D-glucose in tumor cells in vivo and in vitro by 13C nuclear magnetic resonance spectroscopy

Gil Navon; Robbe C. Lyon; Ofer Kaplan; Jack S. Cohen

doi:10.1016/0014-5793(89)81246-3

Monitoring the transport and phosphorylation of 2-deoxy-D-glucose in tumor cells in vivo and in vitro by ¹³C nuclear magnetic resonance spectroscopy

Gil Navon, Robbe C. Lyon, Ofer Kaplan, Jack S. Cohen^*

^*Corresponding author for this work

National Institutes of Health

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

We describe the use of 2-deoxy-D-[6-¹³C]glucose to follow simultaneously, by ¹³C NMR, both transport and phosporylation to its 6-phosphate form, in MCF-7 breast cancer cells in vitro and in vivo in subcutaneous tumors in nude mice.

Original language	English
Pages (from-to)	86-90
Number of pages	5
Journal	FEBS Letters
Volume	247
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(89)81246-3
State	Published - 10 Apr 1989
Externally published	Yes

Keywords

(Cancer cell, Tumor)
Deoxy-D-glucose, 2-
Glycolysis
Labeling, C
NMR, C-
Phosphorylation
Transport

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0014-5793(89)81246-3

Cite this

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abstract = "We describe the use of 2-deoxy-D-[6-13C]glucose to follow simultaneously, by 13C NMR, both transport and phosporylation to its 6-phosphate form, in MCF-7 breast cancer cells in vitro and in vivo in subcutaneous tumors in nude mice.",

keywords = "(Cancer cell, Tumor), Deoxy-D-glucose, 2-, Glycolysis, Labeling, C, NMR, C-, Phosphorylation, Transport",

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AU - Lyon, Robbe C.

AU - Kaplan, Ofer

AU - Cohen, Jack S.

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KW - Glycolysis

KW - Labeling, C

KW - NMR, C-

KW - Phosphorylation

KW - Transport

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