Monitoring the ATM-mediated DNA damage response in the cerebellum using organotypic cultures

Efrat Tal, Yosef Shiloh*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


The ATM gene and its protein product, the ATM protein kinase, were identified as a result of attempts to understand the molecular basis of the genetic disorder, ataxia-telangiectasia (A-T). The cardinal symptom of A-T is neurodegeneration expressed primarily as progressive cerebellar atrophy. A major tool in the investigation of ATM functions in the cerebellum is cerebellar organotypic cultures, which allow cerebellar slices to live in culture for several weeks without losing their viability and organization. These cultures are amenable to various treatments and manipulations and provide a close look at Purkinje cells in their almost natural environment. We optimized the protocol for establishing and maintaining these cultures and provide here examples of readouts of the DNA damage response in cerebellar organotypic cultures treated with a DNA-damaging agent.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages12
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


FundersFunder number
A-T Ease Foundation
Israel Ministry of Education
Israel Cancer Research Fund
A-T Children's Project
Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
Israel Science Foundation
Israeli Centers for Research Excellence


    • ATM kinase
    • Ataxia-telangiectasia (A-T)
    • Cerebellum
    • DNA damage response (DDR)
    • Organotypic cultures


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