Monitoring phage biopanning by next-generation sequencing

Anna Vaisman-Mentesh, Yariv Wine

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Phage display has enabled the rapid isolation of antigen-specific antibodies from combinatorial libraries of the variable heavy chain gene (VH) and variable light chain gene (VL). The method is based on genetic engineering of bacteriophages and repeated rounds of antigen-guided selection by phage biopanning. Next-Generation Sequencing (NGS) coupled with bioinformatics are powerful tools for analyzing the large number of DNA sequences present in an immune library. Here, we describe a method that demonstrates how NGS analysis enhances phage biopanning of complex antibody libraries as well as facilitates the antibody discovery process.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages463-473
Number of pages11
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1701
ISSN (Print)1064-3745

Keywords

  • Antibody repertoire
  • B-Cell receptor repertoire
  • HTS
  • High-throughput sequencing
  • Immunoglobulin repertoire
  • NGS
  • Next-Generation Sequencing
  • ScFv
  • V gene
  • VDJ
  • Variable region

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