Molecular recognition between aβ-specific single-domain antibody and aβ misfolded aggregates

Mingzhen Zhang, Jie Zheng, Ruth Nussinov, Buyong Ma

Research output: Contribution to journalArticlepeer-review

Abstract

Aβ is the toxic amyloid polypeptide responsible for Alzheimer’s disease (AD). Prevention and elimination of the Aβ misfolded aggregates are the promising therapeutic strategies for the AD treatments. Gammabody, the Aβ-Specific Single-domain (VH) antibody, recognizes Aβ aggregates with high affinity and specificity and reduces their toxicities. Employing the molecular dynamics simulations, we studied diverse gammabody-Aβ recognition complexes to get insights into their structural and dynamic properties and gammabody-Aβ recognitions. Among many heterogeneous binding modes, we focused on two gammabody-Aβ recognition scenarios: recognition through Aβ β-sheet backbone and on sidechain surface. We found that the gammabody primarily uses the complementarity-determining region 3 (CDR3) loop with the grafted Aβ sequence to interact with the Aβ fibril, while CDR1/CDR2 loops have very little contact. The gammabody-Aβ complexes with backbone binding mode are more stable, explaining the gammabody’s specificity towards the C-terminal Aβ sequence.

Original languageEnglish
Article number25
JournalAntibodies
Volume7
Issue number3
DOIs
StatePublished - Sep 2018

Keywords

  • Alzheimer’s disease
  • Amyloid antibody
  • Antibody recognition
  • Aβ peptide
  • Single-domain antibodies

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