Molecular probe for enzymatic activity with dual output

Eyal Danieli, Doron Shabat

Research output: Contribution to journalArticlepeer-review

Abstract

A novel molecular probe for enzymatic activity with a dual output detection-mode has been developed. The probe effectively detected the presence of the bacterial protease penicillin-G-amidase; a single cleavage by the enzyme initiated the fragmentation of a self-immolative dendritic platform to release two reporter units. The signals of the free reporters were detected by two different spectroscopic techniques, fluorescence and UV-vis. This is the first reported molecular probe with two different chromogenic reporter units activated by a specific stimulus.

Original languageEnglish
Pages (from-to)7318-7324
Number of pages7
JournalBioorganic and Medicinal Chemistry
Volume15
Issue number23
DOIs
StatePublished - 1 Dec 2007

Keywords

  • Assay
  • Dendrimer
  • Enzyme
  • Molecular probe
  • Self-immolative

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