Molecular Genotyping of Herbicide Resistance in P. minor: ACCase Resistance

Rajender Singh; Davinder Sharma; Nishu Raghav; Rajender Singh Chhokar; Indu Sharma

doi:10.1007/s12010-014-1363-7

Molecular Genotyping of Herbicide Resistance in P. minor: ACCase Resistance

Rajender Singh^*, Davinder Sharma, Nishu Raghav, Rajender Singh Chhokar, Indu Sharma

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Little seed canary grass (Phalaris minor Retz.) populations resistant to herbicides that inhibit acetyl-CoA carboxylase (ACCase) represent an increasingly important weed control problem in northern India. The objective of this study was to develop DNA-based markers to differentiate herbicide-resistant and herbicide-susceptible population of P. minor. Primers were designed to amplify the conserved region carrying two reported mutations Trp₂₀₂₇ to Cys and Ile₂₀₄₁ to Asn conferring ACCase inhibitor resistance in several grass weeds and subjected to single-strand conformational polymorphism (SSCP) to detect the mutations. Five distinctive electrophoretic patterns on non-denaturing PAGE were observed, and four patterns were found to be associated with ACCase herbicide resistance in P. minor. The PCR-SSCP test developed in this study confirmed 17 resistant populations to contain mutations in CT domain of ACCase gene. This is the first report of rapid and easy molecular diagnosis of ACCase herbicide-resistant and herbicide-sensitive population of P. minor through PCR-SSCP analysis.

Original language	English
Pages (from-to)	1617-1621
Number of pages	5
Journal	Applied Biochemistry and Biotechnology
Volume	175
Issue number	3
DOIs	https://doi.org/10.1007/s12010-014-1363-7
State	Published - Feb 2014
Externally published	Yes

Keywords

Acetyl-CoA carboxylase
Herbicide resistance
Mutation
Phalaris minor
SSCP

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title = "Molecular Genotyping of Herbicide Resistance in P. minor: ACCase Resistance",

abstract = "Little seed canary grass (Phalaris minor Retz.) populations resistant to herbicides that inhibit acetyl-CoA carboxylase (ACCase) represent an increasingly important weed control problem in northern India. The objective of this study was to develop DNA-based markers to differentiate herbicide-resistant and herbicide-susceptible population of P. minor. Primers were designed to amplify the conserved region carrying two reported mutations Trp2027 to Cys and Ile2041 to Asn conferring ACCase inhibitor resistance in several grass weeds and subjected to single-strand conformational polymorphism (SSCP) to detect the mutations. Five distinctive electrophoretic patterns on non-denaturing PAGE were observed, and four patterns were found to be associated with ACCase herbicide resistance in P. minor. The PCR-SSCP test developed in this study confirmed 17 resistant populations to contain mutations in CT domain of ACCase gene. This is the first report of rapid and easy molecular diagnosis of ACCase herbicide-resistant and herbicide-sensitive population of P. minor through PCR-SSCP analysis.",

keywords = "Acetyl-CoA carboxylase, Herbicide resistance, Mutation, Phalaris minor, SSCP",

author = "Rajender Singh and Davinder Sharma and Nishu Raghav and Chhokar, {Rajender Singh} and Indu Sharma",

note = "Publisher Copyright: {\textcopyright} 2014, Springer Science+Business Media New York.",

year = "2014",

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doi = "10.1007/s12010-014-1363-7",

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AU - Sharma, Davinder

AU - Raghav, Nishu

AU - Chhokar, Rajender Singh

AU - Sharma, Indu

PY - 2014/2

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N2 - Little seed canary grass (Phalaris minor Retz.) populations resistant to herbicides that inhibit acetyl-CoA carboxylase (ACCase) represent an increasingly important weed control problem in northern India. The objective of this study was to develop DNA-based markers to differentiate herbicide-resistant and herbicide-susceptible population of P. minor. Primers were designed to amplify the conserved region carrying two reported mutations Trp2027 to Cys and Ile2041 to Asn conferring ACCase inhibitor resistance in several grass weeds and subjected to single-strand conformational polymorphism (SSCP) to detect the mutations. Five distinctive electrophoretic patterns on non-denaturing PAGE were observed, and four patterns were found to be associated with ACCase herbicide resistance in P. minor. The PCR-SSCP test developed in this study confirmed 17 resistant populations to contain mutations in CT domain of ACCase gene. This is the first report of rapid and easy molecular diagnosis of ACCase herbicide-resistant and herbicide-sensitive population of P. minor through PCR-SSCP analysis.

AB - Little seed canary grass (Phalaris minor Retz.) populations resistant to herbicides that inhibit acetyl-CoA carboxylase (ACCase) represent an increasingly important weed control problem in northern India. The objective of this study was to develop DNA-based markers to differentiate herbicide-resistant and herbicide-susceptible population of P. minor. Primers were designed to amplify the conserved region carrying two reported mutations Trp2027 to Cys and Ile2041 to Asn conferring ACCase inhibitor resistance in several grass weeds and subjected to single-strand conformational polymorphism (SSCP) to detect the mutations. Five distinctive electrophoretic patterns on non-denaturing PAGE were observed, and four patterns were found to be associated with ACCase herbicide resistance in P. minor. The PCR-SSCP test developed in this study confirmed 17 resistant populations to contain mutations in CT domain of ACCase gene. This is the first report of rapid and easy molecular diagnosis of ACCase herbicide-resistant and herbicide-sensitive population of P. minor through PCR-SSCP analysis.

KW - Acetyl-CoA carboxylase

KW - Herbicide resistance

KW - Mutation

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Molecular Genotyping of Herbicide Resistance in P. minor: ACCase Resistance

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Keywords

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