TY - JOUR
T1 - Molecular cloning of a novel human gene encoding a 63-kDa protein and its sublocalization within the 11q13 locus
AU - Perelman, Boris
AU - Dafni, Naomi
AU - Naiman, Tova
AU - Eli, Dalia
AU - Yaakov, Miri
AU - Yang Feng, Teresa L.
AU - Sinha, Srish
AU - Weber, Günther
AU - Khodaei, Shideh
AU - Sancar, Aziz
AU - Dotan, Iris
AU - Canaani, Dan
N1 - Funding Information:
We thank Dr. E. Canaani for the gift of the KCL22, K562, and ALL-1 cDNA libraries. We are grateful to Dr. D. Arad for her help and insight in sequence analysis. This work was supported by grants awarded to D.C. by the Public Health Service National Cancer Institute, the Israel Science Foundation, and the United States±Israel Binational Science Foundation, as well as a fellowship from the Israel Cancer Association.
PY - 1997/5/1
Y1 - 1997/5/1
N2 - A human cDNA previously isolated by virtue of its ability to complement partially the ultraviolet sensitivity of a xeroderma pigmentosum cell line was further characterized. The transcription unit is expressed as a single 4.0-kb mRNA that encodes a novel 63-kDa cytoplasmic protein, possibly initiating from an internal AUG codon. The gene encoding this protein, named UVRAG, has been extremely well conserved during evolution, implying an important role for this gene product in cell metabolism. The transcribed mRNA is constitutively expressed in a wide variety of human tissues. The protein encoded by this gene is predicted to contain a coiled-coil structure and is likely to be metabolically unstable based on the occurrence of a strong PEST domain. UVRAG was assigned to human chromosome 11 by Southern hybridization to a somatic cell hybrid panel. Fluorescence in situ hybridization coupled with PCR analysis of human/rodent somatic cell hybrids containing segments of human chromosome 11 has localized this gene to a subregion of 11q13 in between the D11S916 and the D11S906 loci. Importantly, this region has been shown to be amplified in a variety of human malignancies, including breast cancer.
AB - A human cDNA previously isolated by virtue of its ability to complement partially the ultraviolet sensitivity of a xeroderma pigmentosum cell line was further characterized. The transcription unit is expressed as a single 4.0-kb mRNA that encodes a novel 63-kDa cytoplasmic protein, possibly initiating from an internal AUG codon. The gene encoding this protein, named UVRAG, has been extremely well conserved during evolution, implying an important role for this gene product in cell metabolism. The transcribed mRNA is constitutively expressed in a wide variety of human tissues. The protein encoded by this gene is predicted to contain a coiled-coil structure and is likely to be metabolically unstable based on the occurrence of a strong PEST domain. UVRAG was assigned to human chromosome 11 by Southern hybridization to a somatic cell hybrid panel. Fluorescence in situ hybridization coupled with PCR analysis of human/rodent somatic cell hybrids containing segments of human chromosome 11 has localized this gene to a subregion of 11q13 in between the D11S916 and the D11S906 loci. Importantly, this region has been shown to be amplified in a variety of human malignancies, including breast cancer.
UR - http://www.scopus.com/inward/record.url?scp=0031149239&partnerID=8YFLogxK
U2 - 10.1006/geno.1997.4623
DO - 10.1006/geno.1997.4623
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C2 - 9169138
AN - SCOPUS:0031149239
SN - 0888-7543
VL - 41
SP - 397
EP - 405
JO - Genomics
JF - Genomics
IS - 3
ER -
