Molecular characterization of a salt-tolerant bacterial community in the rice rhizosphere

Anil K. Tripathi*, Subhash C. Verma, Eliora Z. Ron

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

The diversity of salt-tolerant bacteria present in the rhizosphere of Oryza sativa was investigated. Fourteen bacterial strains, isolated after enrichment in nitrogen-free, semi-solid medium and showing tolerance to 3% NaCl, were analyzed by restriction patterns produced by amplified DNA coding for 16S rDNA (ARDRA) with enzymes Sau3AI, AluI and RsaI which showed that they were represented by 4 ARDRA types. Biodiversity among the 14 strains was also analyzed by the random amplified polymorphic DNA (RAPD) technique with a 10-mer primer. Partial nucleotide sequence of 16S rDNA assigned these clusters to Serratia marcescens, Pseudomonas aeruginosa, Alcaligenes xylosoxidans and Ochrobactrum anthropi. Notably, all four bacterial species are potential human pathogens that infect immunocompromised patients.

Original languageEnglish
Pages (from-to)579-584
Number of pages6
JournalResearch in Microbiology
Volume153
Issue number9
DOIs
StatePublished - Nov 2002

Funding

FundersFunder number
Ministry of Environment and Forests, Government of India

    Keywords

    • ARDRA
    • Human pathogens
    • Rice rhizosphere
    • Salt-tolerant bacteria

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