Molecular analysis of the DNA gyrB gene from Myxococcus xanthus

Yossi Paitan, Neil Boulton, Eliora Z. Ron, Eugene Rosenberg, Elisha Orr

Research output: Contribution to journalArticlepeer-review

Abstract

DNA gyrase, an essential type II topoisomerase, mediates negative supercoiling of the bacterial chromosome, thereby affecting the processes of DNA replication, transcription, recombination and repair. The gyrB gene from the Gram-negative soil bacterium Myxococcus xanthus was sequenced. The sequence predicts a protein of 815 amino acid residues displaying significant homology to all known GyrB proteins. A 6-His-GyrB fusion protein was overexpressed in Escherichia coli and purified to near homogeneity using affinity chromatography on Ni-nitrilotriacetic acid-agarose and novobiocin-Sepharose columns. The fusion protein bound novobiocin and cross-reacted with anti-E. coli GyrB antibodies, indicating structural and functional similarities to the E. coli DNA GyrB. The gene was mapped to the region of the origin of replication (oriC) of M. xanthus.

Original languageEnglish
Pages (from-to)1641-1647
Number of pages7
JournalMicrobiology
Volume144
Issue number6
DOIs
StatePublished - Jun 1998

Keywords

  • DNA gyrase B
  • Myxococcus xanthus
  • Topoisomerase

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