Molecular analysis of recombinase-mediated cassette exchange reactions catalyzed by integrase of coliphage HK022

Natalia Malchin, Tatiana Molotsky, Ezra Yagil, Alexander B. Kotlyar, Mikhail Kolot*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The integrase (Int) protein of coliphage HK022 can catalyze in Escherichia coli as well as in in vitro integrative and excisive recombinase-mediated cassette exchange reactions between plasmids as substrates. Atomic force microscopy images have revealed that in the protein-DNA complexes that are formed, the plasmid substrates are connected via one and not two pairs of attachment sites. This observation, together with the elucidation of intermediate co-integrates between the two circular plasmids, suggest that a sequential mechanism of the RMCE reaction is possible.

Original languageEnglish
Pages (from-to)663-670
Number of pages8
JournalResearch in Microbiology
Volume159
Issue number9-10
DOIs
StatePublished - Nov 2008

Funding

FundersFunder number
European grant for Future and Emerging TechnologiesFP6-029192
United States-Israel Binational Science Foundation2003304

    Keywords

    • Atomic force microscopy
    • Bacteriophage HK022
    • Integrase
    • Recombinase-mediated cassette exchange
    • Site-specific recombination

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