@article{ba803d745e1146cf971d133db32067dc,
title = "Molecular analysis of cDNA and the transcript encoding the depressant insect selective neurotoxin of the scorpion Leiurus quinquestriatus hebraeus",
abstract = "Analysis of mRNA transcribed off the gene encoding the depressant insect selective neurotoxin (LqhIT2) of the scorpion Leiurus quinquestriatus hebraues revealed a transcript about 400 nucleotides long and two putative precursors of about 4.4 and 3.5 kb long. Poly(A)+ RNA, purified from the venom-gland segments of the scorpion, was reverse transcribed into cDNA and a cDNA library was constructed. Two closely related cDNA clones were isolated from the library by probing with a partial cDNA clone encoding the depressant insect toxin of the scorption Buthotus judaicus. Sequence analysis of the two LqhIT2 cDNA clones revealed an identical 63 bp leader sequence, a 183 bp sequence encoding the mature polypeptide, and 9 bp encoding Gly-Lys-Lys prior to the termination codon. The two clones are distinguished by a single base substitution at the codon corresponding to amino acid residue 16 of the mature toxin, and at the non-coding region preceding the poly(A) tail. These findings elucidate the processing steps in the maturation of LqhIT2 and indicate genetic polymorphism among the genes encoding this toxin.",
keywords = "cDNA, scorpion-neurotoxin, sequencing, transcription",
author = "Noam Zilberberg and Eliahu Zlotkin and Michael Gurevitz",
note = "Funding Information: Acknowledgements-We thank Dr D. Gordon (Hebrew University, Jerusalem) for help and advice, and the Photography Unit of Tel-Aviv University for their service. This research was supported in part by the “Conservation, Food and Health Foundation, Inc.“, Boston, Mass., U.S.A.",
year = "1992",
month = mar,
doi = "10.1016/0965-1748(92)90160-G",
language = "אנגלית",
volume = "22",
pages = "199--203",
journal = "Insect Biochemistry and Molecular Biology",
issn = "0965-1748",
publisher = "Elsevier Ltd.",
number = "2",
}