Modulatory effect of agents active in the presynaptic dopaminergic system on the striatal dopamine transporter

Irit Gordon, Ronit Weizman, Moshe Rehavi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We have investigated the effects of agents active in the presynaptic dopaminergic system on the characterization of the rat striatal dopamine transporter. The dopamine transporter was characterized by high-affinity [3H]GBR 12935 (1-[2-diphenylmethoxy)-ethyl]-4-(3-phenylpropyl)-piperazine) binding to a membrane preparation and by [3H]dopamine uptake into striatal synaptosomes. Subchronic treatment with reserpine (2.5 mg/kg, 4 days), a monoamine depletor, caused a significant decrease in both [3H]GBR 12935 binding (20%) and [3H]dopamine uptake (51%). In contrast, amantadine (a dopamine releaser) treatment (20 mg/kg, 21 days) induced an increase (28%) in the maximal number of [3H]GBR 12935 sites. Chronic levo-dopa (dopamine precursor) treatment combined with carbidopa (50 mg/kg and 5 mg/kg respectively, 21 days) as well as benztropine (dopamine uptake inhibitor) treatment (10 mg/kg, 21 days) did not affect the striatal dopamine transporter characteristics. The present results showed that the striatal dopamine transporter is sensitive to changes in dopaminergic neurotransmission caused by agents that do not interact directly with the dopamine carrier.

Original languageEnglish
Pages (from-to)27-30
Number of pages4
JournalEuropean Journal of Pharmacology
Volume298
Issue number1
DOIs
StatePublished - 29 Feb 1996

Keywords

  • Amantadine
  • Benztropine
  • Dopamine transporter
  • L-DOPA
  • Reserpine
  • [H]GBR 12935 binding

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