Modulation of vertebrate brain Na+ and K+ channels by subtypes of protein kinase C

Ilana Lotan*, Nathan Dascal, Zvi Naor, Rony Boton

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Effects of purified subtypes I, II and III of protein kinase C (PKC) on voltage-dependent transient K+ (A) and Na+ channels were studied in Xenopus oocytes injected with chick brain RNA. The experiments were performed in the constant presence of 10 nM β-phorbol 12-myristate-13-acetate (PMA). Intracellular injection of subtype I (τ) reduced the A-current (IA), with no effect on Na+ current (INa). PKC subtype II (β1, + β2) and III (α) reduced both currents. PKC did not affect the response to kainate. Inactivated (heated) or unactivated (injected in the absence of PMA) enzyme and vehicle alone had no effect. Our results strongly suggest that INa, and IA in vertebrate neurons are modulated by PKC; all PKC subtypes exert a similar effect on the A-channel while only subtypes II and III modulate the Na+ channel.

Original languageEnglish
Pages (from-to)25-28
Number of pages4
JournalFEBS Letters
Volume267
Issue number1
DOIs
StatePublished - 2 Jul 1990

Funding

FundersFunder number
US-Israel Binational Science Foundation
Bloom's Syndrome Foundation
Israel Academy of Sciences and Humanities

    Keywords

    • A channel
    • Modulation
    • Oocyte
    • Protein kinase C isoform
    • RNA expression
    • Sodium channel
    • Xenopus

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