TY - JOUR
T1 - Modulation of tissue plasminogen activator biosynthesis by phosphatidylinositol liposomes in human fetal lung fibroblasts
AU - Floru, S.
AU - Gelvan, A.
AU - Maran, R.
AU - Kadouri, A.
AU - Cohen, A. M.
PY - 1991/2
Y1 - 1991/2
N2 - Phosphatidylinositol (PI) liposomes at 40 μM increased tissue plasminogen activator (t‐PA) biosynthesis by human fetal lung fibroblasts IMR‐90 (FLF), after 5 days of incubation by 7.4 ± 1.4 times of the control level. Other phospholipid liposomes, such as phosphatidylserine (PS), phosphatidylcholine (PC), and phosphatidylglycerol (PG), had no effect on t‐PA biosynthesis by FLF. The induction of t‐PA biosynthesis by PI liposomes was inhibited by specific inhibitors of phosphoinositide pathway: gentamycin and lithium chloride. Thus, gentamycin inhibited the effect of PI liposomes on t‐PA biosynthesis by 76% (P < 0.001), while it had no effect on control FLF. Likewise, lithium chloride inhibited t‐PA biosynthesis of both PI‐treated and control FLF by >84%. The induction of t‐PA biosynthesis by PI liposomes was dependent on RNA transcription and independent of DNA biosynthesis.
AB - Phosphatidylinositol (PI) liposomes at 40 μM increased tissue plasminogen activator (t‐PA) biosynthesis by human fetal lung fibroblasts IMR‐90 (FLF), after 5 days of incubation by 7.4 ± 1.4 times of the control level. Other phospholipid liposomes, such as phosphatidylserine (PS), phosphatidylcholine (PC), and phosphatidylglycerol (PG), had no effect on t‐PA biosynthesis by FLF. The induction of t‐PA biosynthesis by PI liposomes was inhibited by specific inhibitors of phosphoinositide pathway: gentamycin and lithium chloride. Thus, gentamycin inhibited the effect of PI liposomes on t‐PA biosynthesis by 76% (P < 0.001), while it had no effect on control FLF. Likewise, lithium chloride inhibited t‐PA biosynthesis of both PI‐treated and control FLF by >84%. The induction of t‐PA biosynthesis by PI liposomes was dependent on RNA transcription and independent of DNA biosynthesis.
KW - Phosphatidylinositol
KW - cell culture
KW - fibroblasts
KW - tissue plasminogen activator
UR - http://www.scopus.com/inward/record.url?scp=0026030054&partnerID=8YFLogxK
U2 - 10.1002/ajh.2830360207
DO - 10.1002/ajh.2830360207
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AN - SCOPUS:0026030054
VL - 36
SP - 100
EP - 104
JO - American Journal of Hematology
JF - American Journal of Hematology
SN - 0361-8609
IS - 2
ER -