Modulation of tissue plasminogen activator biosynthesis by phosphatidylinositol liposomes in human fetal lung fibroblasts

S. Floru, A. Gelvan, R. Maran, A. Kadouri, A. M. Cohen

Research output: Contribution to journalArticlepeer-review

Abstract

Phosphatidylinositol (PI) liposomes at 40 μM increased tissue plasminogen activator (t‐PA) biosynthesis by human fetal lung fibroblasts IMR‐90 (FLF), after 5 days of incubation by 7.4 ± 1.4 times of the control level. Other phospholipid liposomes, such as phosphatidylserine (PS), phosphatidylcholine (PC), and phosphatidylglycerol (PG), had no effect on t‐PA biosynthesis by FLF. The induction of t‐PA biosynthesis by PI liposomes was inhibited by specific inhibitors of phosphoinositide pathway: gentamycin and lithium chloride. Thus, gentamycin inhibited the effect of PI liposomes on t‐PA biosynthesis by 76% (P < 0.001), while it had no effect on control FLF. Likewise, lithium chloride inhibited t‐PA biosynthesis of both PI‐treated and control FLF by >84%. The induction of t‐PA biosynthesis by PI liposomes was dependent on RNA transcription and independent of DNA biosynthesis.

Original languageEnglish
Pages (from-to)100-104
Number of pages5
JournalAmerican Journal of Hematology
Volume36
Issue number2
DOIs
StatePublished - Feb 1991

Keywords

  • Phosphatidylinositol
  • cell culture
  • fibroblasts
  • tissue plasminogen activator

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