Modulation of the response to estradiol-17β of rat vascular tissues by a non calcemic vitamin D analog

Dalia Somjen*, Sara Katzburg, Merav Baz, Naftali Stern, Gary H. Posner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Estradiol-17β (E2) increases creatine kinase (CK) specific activity in aorta (Ao) and left ventricle of the heart (Lv) from rat females. In the present study, we analyzed the effects of pretreatment with the non calcemic analog of vitamin D, JK 1624 F2-2 (JKF) on the response to E 2 (either 0.5 or 5 μg/rat) of Ao and Lv from prepubertal female rats. JKF did not affect CK in either organ. However, pretreatment with JKF (0.1 ng/g body weight for 1 or 2 weeks) increased the CK response to E2 (0.5 μg/rat) by 50±10% in Ao and by 150±12% in Lv. The CK response to 5 μg/rat of E2 in intact female rats, was increased by 118±15% and 99±11% in the Ao and by 89±6% and 112±13% in the Lv, in animals treated daily with JKF for 1 or 2 weeks, respectively, before administration of E2. JKF also increased the response to 500 μg/rat raloxifene (Ral) by 47±8% in Ao and by 56±12% in Lv. Preliminary experiments showed that JKF treatment induced a ∼50% increase in estradiol receptor ERα in both organs. The results indicate that the vitamin D analog JKF upregulates the response and sensitivity of vascular tissues to E2, in association with increased expression of their ERα. These results should prompt examination of the possibility that the effects estrogen therapy in postmenopausal women can be augmented by vitamin D or its analogs.

Original languageEnglish
Pages (from-to)339-341
Number of pages3
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume89-90
DOIs
StatePublished - May 2004
Externally publishedYes

Keywords

  • "Non hypercalcemic" vitamin D analogs
  • Creatine kinase
  • Estrogen
  • Estrogen receptors α and β

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