Modulation of Alzheimer's β-amyloid neurotoxicity by site-directed single-chain antibody

Research output: Contribution to journalArticlepeer-review

Abstract

A single-chain antibody was constructed from variable regions of heavy and light genes of the parental anti-β-amyloid peptide IgM 508 antibody. This antibody exhibits anti-aggregating properties, leading to disaggregation of Alzheimer β-amyloid (βA) fibrils and prevents its toxic effect on cultured PC-12 cells. Sequencing of the small antibody, namely 508 (Fv), revealed that the V(L) domain contained a cysteine residue in the complementary determining region (CDR)3 (residue 96) which affects its solubility and stability. The cysteine codon was replaced using SOE PCR, and one of the mutants obtained, namely 508F(Fv) (containing phenylalanine instead of cysteine), showed an increased storage stability and higher affinity compared to the wild type. Antibody 508F(Fv) prevents the neurotoxicity of βA (90% cell viability) and disrupts the fibril structure of β-amyloid (62% decrease in ThT fluorescence). The ability of antibody 508F(Fv) to dissolve already-formed βA fibrils makes it a good candidate for intracellular expression and modulation of APP processing as the first step towards the production of therapeutic protection molecules for Alzheimer's disease treatment. (C) 2000 Published by Elsevier Science B.V.

Original languageEnglish
Pages (from-to)23-31
Number of pages9
JournalJournal of Neuroimmunology
Volume106
Issue number1-2
DOIs
StatePublished - 1 Jul 2000

Keywords

  • Engineered antibodies
  • Neurotoxicity
  • β-Amyloid modulation

Fingerprint

Dive into the research topics of 'Modulation of Alzheimer's β-amyloid neurotoxicity by site-directed single-chain antibody'. Together they form a unique fingerprint.

Cite this