Modulation of aggregation propensity of Aβ38 by site specific multiple proline substitution

Nadimpally Krishna Chaitanya, Ashim Paul, Abhijit Saha, Bhubaneswar Mandal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Proline with its unique geometrical feature generates turn in the peptide backbone. Therefore, aggregation potential of Aβ38 can be modulated by insertion of proline at specific positions. Although site specific proline mutation is reported, effect of multiple proline substitution on aggregation potential is still not demonstrated. Therefore, Aβ38, a single proline substituted variant, V18P-Aβ38, a double proline substituted variant, V18P-I31P-Aβ38 and a triple proline substituted version, V12P-V18P-I31P-Aβ38 were synthesized and their aggregation potential were studied. The proline mutants found to have higher solubility and slower aggregation kinetics. The double mutated version was relatively less aggregation prone than its single mutated version. Such analogues can be useful for designing new β sheet breaker peptides, studying the mechanism of aggregation and structural analyses.

Original languageEnglish
Pages (from-to)365-371
Number of pages7
JournalInternational Journal of Peptide Research and Therapeutics
Issue number4
StatePublished - Dec 2013
Externally publishedYes


  • Aggregation
  • Alzheimer's disease
  • Amyloid β peptide
  • Proline substitution


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