TY - JOUR
T1 - Modification of histidine residues by diethyl pyrocarbonate leads to inactivation of the Rhodospirillum rubrum RrF1-ATPase
AU - Khananshvili, Daniel
AU - Zippora, Gromet Elhanan
PY - 1983/8/8
Y1 - 1983/8/8
N2 - The RrF1-ATPase from the photosynthetic bacterium Rhodospirillum rubrum was rapidly and completely inactivated by diethyl pyrocarbonate (DEPC) at pH 6.0 and 22°C. When applied in 1 step 5 mM DEPC were required for > 90% inactivation and this DEPC-modified enzyme showed an increase in absorption at 242 nm as well as a decrease in absorption at 280 nm, suggesting modification of both histidine and tyrosine residues. Complete inactivation of the RrF1-ATPase could be obtained with only 250 μM DEPC when applied in 5 separate steps of 50 μM each. The only absorption change observed under these conditions was an increase at 242 nm indicating that the inactivation can be correlated with modification of histidine residues. Complete inactivation requires the modification of 2-3 histidine residues per molecule of RrF1.
AB - The RrF1-ATPase from the photosynthetic bacterium Rhodospirillum rubrum was rapidly and completely inactivated by diethyl pyrocarbonate (DEPC) at pH 6.0 and 22°C. When applied in 1 step 5 mM DEPC were required for > 90% inactivation and this DEPC-modified enzyme showed an increase in absorption at 242 nm as well as a decrease in absorption at 280 nm, suggesting modification of both histidine and tyrosine residues. Complete inactivation of the RrF1-ATPase could be obtained with only 250 μM DEPC when applied in 5 separate steps of 50 μM each. The only absorption change observed under these conditions was an increase at 242 nm indicating that the inactivation can be correlated with modification of histidine residues. Complete inactivation requires the modification of 2-3 histidine residues per molecule of RrF1.
KW - Bacterial ATPase
KW - Diethyl pyrocarbonate
KW - Histidine residue
KW - Inhibition of ATPase
KW - Rhodospirillum rubrum
UR - http://www.scopus.com/inward/record.url?scp=0004801533&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(83)80462-1
DO - 10.1016/0014-5793(83)80462-1
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AN - SCOPUS:0004801533
VL - 159
SP - 271
EP - 274
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -