@article{b0c866882baf4a268309b8c27e4a3bac,
title = "Modeling sars-cov-2 infection in mice using lentiviral hace2 vectors infers two modes of immune responses to sars-cov-2 infection",
abstract = "Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a severe global pandemic. Mice models are essential to investigate infection pathology, antiviral drugs, and vaccine development. However, wild-type mice lack the human angiotensin-converting enzyme 2 (hACE2) that mediates SARS-CoV-2 entry into human cells and consequently are not susceptible to SARS-CoV-2 infection. hACE2 transgenic mice could provide an efficient COVID-19 model, but are not always readily available, and practically restricted to specific strains. Therefore, there is a dearth of additional mouse models for SARS-CoV-2 infection. We applied lentiviral vectors to generate hACE2 expression in interferon receptor knock-out (IFNAR1−/−) mice. Lenti-hACE2 transduction supported SARS-CoV-2 replication in vivo, simulating mild acute lung disease. Gene expression analysis revealed two modes of immune responses to SARS-CoV-2 infection: one in response to the exposure of mouse lungs to SARS-CoV-2 particles in the absence of productive viral replication, and the second in response to productive SARS-CoV-2 infection. Our results infer that immune response to immunogenic elements on incoming virus or in productively infected cells stimulate diverse immune effectors, even in absence of type I IFN signaling. Our findings should contribute to a better understanding of the immune response triggered by SARS-CoV-2 and to further elucidate COVID-19.",
keywords = "COVID-19, HACE2, Immune response, Lentivirus, Mouse model, SARS-CoV-2",
author = "Chaja Katzman and Tomer Israely and Sharon Melamed and Boaz Politi and Assa Sittner and Yfat Yahalom-Ronen and Shay Weiss and Rass, {Reem Abu} and Rachel Zamostiano and Eran Bacharach and Marcelo Ehrlich and Nir Paran and Lior Nissim",
note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
month = jan,
doi = "10.3390/v14010011",
language = "אנגלית",
volume = "14",
journal = "Viruses",
issn = "1999-4915",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "1",
}