Mitochondrial function and tissue vitality: Bench-to-bedside real-time optical monitoring system

Avraham Mayevsky, Raphael Walden, Eliyahu Pewzner, Assaf Deutsch, Eitan Heldenberg, Jacob Lavee, Salis Tager, Erez Kachel, Ehud Raanani, Sergey Preisman, Violete Glauber, Eran Segalf

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The involvement of mitochondria in pathological states, such as neurodegenerative diseases, sepsis, stroke, and cancer, are well documented. Monitoring of nicotinamide adenine dinucleotide (NADH) fluorescence in vivo as an intracellular oxygen indicator was established in 1950 to 1970 by Britton Chance and collaborators. We use a multiparametric monitoring system enabling assessment of tissue vitality. In order to use this technology in clinical practice, the commercial developed device, the CritiView (CRV), is tested in animal models as well as in patients. Methods and Results: The new CRV enables the optical monitoring of four different parameters, representing the energy balance of various tissues in vivo. Mitochondrial NADH is measured by surface fluorometry/reflectometry. In addition, tissue microcirculatory blood flow, tissue reflectance and oxygenation are measured as well. The device is tested both in vitro and in vivo in a small animal model and in preliminary clinical trials in patients undergoing vascular or open heart surgery. In patients, the monitoring is started immediately after the insertion of a three-way Foley catheter (urine collection) to the patient and is stopped when the patient is discharged from the operating room. The results show that monitoring the urethral wall vitality provides information in correlation to the surgical procedure performed.

Original languageEnglish
Article number067004
JournalJournal of Biomedical Optics
Volume16
Issue number6
DOIs
StatePublished - Jun 2011
Externally publishedYes

Keywords

  • Biomedical optics
  • Biophotonics
  • Laser doppler velocimetry
  • Reflectometry

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