TY - JOUR
T1 - Mitochondrial function and tissue vitality
T2 - Bench-to-bedside real-time optical monitoring system
AU - Mayevsky, Avraham
AU - Walden, Raphael
AU - Pewzner, Eliyahu
AU - Deutsch, Assaf
AU - Heldenberg, Eitan
AU - Lavee, Jacob
AU - Tager, Salis
AU - Kachel, Erez
AU - Raanani, Ehud
AU - Preisman, Sergey
AU - Glauber, Violete
AU - Segalf, Eran
N1 - Funding Information:
This study was supported by CritiSense Ltd. that had developed the CritiView.
PY - 2011/6
Y1 - 2011/6
N2 - Background: The involvement of mitochondria in pathological states, such as neurodegenerative diseases, sepsis, stroke, and cancer, are well documented. Monitoring of nicotinamide adenine dinucleotide (NADH) fluorescence in vivo as an intracellular oxygen indicator was established in 1950 to 1970 by Britton Chance and collaborators. We use a multiparametric monitoring system enabling assessment of tissue vitality. In order to use this technology in clinical practice, the commercial developed device, the CritiView (CRV), is tested in animal models as well as in patients. Methods and Results: The new CRV enables the optical monitoring of four different parameters, representing the energy balance of various tissues in vivo. Mitochondrial NADH is measured by surface fluorometry/reflectometry. In addition, tissue microcirculatory blood flow, tissue reflectance and oxygenation are measured as well. The device is tested both in vitro and in vivo in a small animal model and in preliminary clinical trials in patients undergoing vascular or open heart surgery. In patients, the monitoring is started immediately after the insertion of a three-way Foley catheter (urine collection) to the patient and is stopped when the patient is discharged from the operating room. The results show that monitoring the urethral wall vitality provides information in correlation to the surgical procedure performed.
AB - Background: The involvement of mitochondria in pathological states, such as neurodegenerative diseases, sepsis, stroke, and cancer, are well documented. Monitoring of nicotinamide adenine dinucleotide (NADH) fluorescence in vivo as an intracellular oxygen indicator was established in 1950 to 1970 by Britton Chance and collaborators. We use a multiparametric monitoring system enabling assessment of tissue vitality. In order to use this technology in clinical practice, the commercial developed device, the CritiView (CRV), is tested in animal models as well as in patients. Methods and Results: The new CRV enables the optical monitoring of four different parameters, representing the energy balance of various tissues in vivo. Mitochondrial NADH is measured by surface fluorometry/reflectometry. In addition, tissue microcirculatory blood flow, tissue reflectance and oxygenation are measured as well. The device is tested both in vitro and in vivo in a small animal model and in preliminary clinical trials in patients undergoing vascular or open heart surgery. In patients, the monitoring is started immediately after the insertion of a three-way Foley catheter (urine collection) to the patient and is stopped when the patient is discharged from the operating room. The results show that monitoring the urethral wall vitality provides information in correlation to the surgical procedure performed.
KW - Biomedical optics
KW - Biophotonics
KW - Laser doppler velocimetry
KW - Reflectometry
UR - http://www.scopus.com/inward/record.url?scp=80455137244&partnerID=8YFLogxK
U2 - 10.1117/1.3585674
DO - 10.1117/1.3585674
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AN - SCOPUS:80455137244
SN - 1083-3668
VL - 16
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
IS - 6
M1 - 067004
ER -