Misidentification of Candida dubliniensis isolates with the VITEK MS

Maya Korem; Matan Joel Cohen; Ayelet Michael-Gayego; Dafna Castiel; Marc V. Assous; Sharon Amit

doi:10.1016/j.mycmed.2020.101107

Misidentification of Candida dubliniensis isolates with the VITEK MS

Maya Korem^*, Matan Joel Cohen, Ayelet Michael-Gayego, Dafna Castiel, Marc V. Assous, Sharon Amit

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The phylogenetic relatedness of Candida dubliniensis and C. albicans may lead to misidentification of C. dubliniensis and underestimation of its clinical significance. We evaluated the performance of VITEK-MS in identifying C. dubliniensis isolates following growth on different culture media. Correct identification was documented in 98% of the isolates grown on blood agar media whereas only 44% were correctly identified from SDA or CHROMagar. The use of non-manufacturer validated media for identifying C. dubliniensis with VITEK-MS, may result in misidentification of these isolates as C. albicans. This finding calls for reassessing the accuracy of fungal isolates identification in local workflows using non-validated culture media.

Original language	English
Article number	101107
Journal	Journal of Medical Mycology
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/j.mycmed.2020.101107
State	Published - Mar 2021
Externally published	Yes

Keywords

CHROMagar
Candida albicans
Candida dubliniensis
MALDI-TOF MS
VITEK MS

Access to Document

10.1016/j.mycmed.2020.101107

Cite this

@article{802aa2f0746648b9afa65987dc479ca7,

title = "Misidentification of Candida dubliniensis isolates with the VITEK MS",

abstract = "The phylogenetic relatedness of Candida dubliniensis and C. albicans may lead to misidentification of C. dubliniensis and underestimation of its clinical significance. We evaluated the performance of VITEK-MS in identifying C. dubliniensis isolates following growth on different culture media. Correct identification was documented in 98% of the isolates grown on blood agar media whereas only 44% were correctly identified from SDA or CHROMagar. The use of non-manufacturer validated media for identifying C. dubliniensis with VITEK-MS, may result in misidentification of these isolates as C. albicans. This finding calls for reassessing the accuracy of fungal isolates identification in local workflows using non-validated culture media.",

keywords = "CHROMagar, Candida albicans, Candida dubliniensis, MALDI-TOF MS, VITEK MS",

author = "Maya Korem and Cohen, {Matan Joel} and Ayelet Michael-Gayego and Dafna Castiel and Assous, {Marc V.} and Sharon Amit",

note = "Publisher Copyright: {\textcopyright} 2020",

year = "2021",

month = mar,

doi = "10.1016/j.mycmed.2020.101107",

language = "אנגלית",

volume = "31",

journal = "Journal of Medical Mycology",

issn = "1156-5233",

publisher = "Elsevier Masson s.r.l.",

number = "1",

}

TY - JOUR

T1 - Misidentification of Candida dubliniensis isolates with the VITEK MS

AU - Korem, Maya

AU - Cohen, Matan Joel

AU - Michael-Gayego, Ayelet

AU - Castiel, Dafna

AU - Assous, Marc V.

AU - Amit, Sharon

PY - 2021/3

Y1 - 2021/3

N2 - The phylogenetic relatedness of Candida dubliniensis and C. albicans may lead to misidentification of C. dubliniensis and underestimation of its clinical significance. We evaluated the performance of VITEK-MS in identifying C. dubliniensis isolates following growth on different culture media. Correct identification was documented in 98% of the isolates grown on blood agar media whereas only 44% were correctly identified from SDA or CHROMagar. The use of non-manufacturer validated media for identifying C. dubliniensis with VITEK-MS, may result in misidentification of these isolates as C. albicans. This finding calls for reassessing the accuracy of fungal isolates identification in local workflows using non-validated culture media.

AB - The phylogenetic relatedness of Candida dubliniensis and C. albicans may lead to misidentification of C. dubliniensis and underestimation of its clinical significance. We evaluated the performance of VITEK-MS in identifying C. dubliniensis isolates following growth on different culture media. Correct identification was documented in 98% of the isolates grown on blood agar media whereas only 44% were correctly identified from SDA or CHROMagar. The use of non-manufacturer validated media for identifying C. dubliniensis with VITEK-MS, may result in misidentification of these isolates as C. albicans. This finding calls for reassessing the accuracy of fungal isolates identification in local workflows using non-validated culture media.

KW - CHROMagar

KW - Candida albicans

KW - Candida dubliniensis

KW - MALDI-TOF MS

KW - VITEK MS

UR - http://www.scopus.com/inward/record.url?scp=85098670347&partnerID=8YFLogxK

U2 - 10.1016/j.mycmed.2020.101107

DO - 10.1016/j.mycmed.2020.101107

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 33388671

AN - SCOPUS:85098670347

SN - 1156-5233

VL - 31

JO - Journal of Medical Mycology

JF - Journal of Medical Mycology

IS - 1

M1 - 101107

ER -

Misidentification of Candida dubliniensis isolates with the VITEK MS

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this