MiR-142 orchestrates a network of actin cytoskeleton regulators during megakaryopoiesis

Elik Chapnik, Natalia Rivkin, Alexander Mildner, Gilad Beck, Ronit Pasvolsky, Eyal Metzl-Raz, Yehudit Birger, Gail Amir, Itay Tirosh, Ziv Porat, Liron Israel, Emmanuel Lellouche, Shulamit Michaeli, Jean Paul Lellouche, Shai Izraeli, Steffen Jung, Eran Hornstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Genome-encoded microRNAs (miRNAs) provide a posttranscriptional regulatory layer that controls the differentiation and function of various cellular systems, including hematopoietic cells. miR-142 is one of the most prevalently expressed miRNAs within the hematopoietic lineage. To address the in vivo functions of miR-142 we utilized a novel reporter and loss-of-function mouse allele that we have recently generated. Here, we show that miR-142 is broadly expressed in the adult hematopoietic system. Our data further reveal that miR-142 is critical for megakaryopoiesis. Thus, genetic miR-142 ablation caused impaired megakaryocyte maturation, inhibition of polyploidization, abnormal proplatelet formation, and thrombocytopenia. Finally, we characterize a network of miR-142-3p targets which collectively controls actin filament homeostasis, thereby ensuring proper execution of actin-dependent proplatelet formation. Our study reveals a pivotal role for miR-142 activity in megakaryocyte maturation and function, and demonstrates a critical contribution of a single miRNA in orchestrating cytoskeletal dynamics and normal haemostasis.

Original languageEnglish
Article numbere01964
Issue number3
StatePublished - 23 May 2014


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