Mimicry by cytokinin of phytochrome‐regulated inhibition of chloroplast development in etiolated cucumber cotyledons

Lila Cohen, Tova Arzee, Aviah Zilberstein

Research output: Contribution to journalArticlepeer-review

Abstract

A study of the kinetics of chlorophyll (Chl) synthesis in cotyledons of etiolated cucumber seedlings (Cucumis sativus L. cv. Delilah) treated with 5×10‐5M ‐ben‐zyladenine (BA) showed that cytokinin, like a red light pulse, could inhibit as well as promote pigment accumulation depending on the length of the dark period following induction. Spraying intact, dark‐grown seedlings with BA, 24 h prior to white light exposure, eliminated the lag phase in Chl synthesis, while treatment with hormone 72 h before greening not only delayed the onset of synthesis, but it also reduced the amount of Chl accumulated after 24 h continuous white light. Impairment of Chl formation was correlated with inhibited regeneration of protochlorophyll and delayed appearance of the light harvesting Chl alb polypeptide. Application of σ‐aminolevulinic acid (15 mM) 2 h before white light exposure shortened the lag phase in Chl synthesis in control as well as in inhibited cotyledons, but the adverse effect of the red light and BA treatments on long‐term Chl accumulation (24 h) was not reversed. Application of glutamate did not stimulate Chl production. Simultaneous treatment with hormone and red light 72 h before greening enhanced their separate inhibitory effects on Chl synthesis, but when given together 24 h prior to white light, their promotive effects on pigment accumulation were not additive.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalPhysiologia Plantarum
Volume72
Issue number1
DOIs
StatePublished - Jan 1988

Keywords

  • BA
  • Cucumis sativis
  • benzyladenine
  • chloroplast
  • cotyledon
  • cucumber
  • cytokinin
  • greening
  • light harvesting chlorophyll alb polypeptide
  • phytochrome
  • protochlorophyll
  • σ‐aminolevulinic acid

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