TY - JOUR
T1 - Mimicking the Brain Extracellular Matrix in Vitro
T2 - A Review of Current Methodologies and Challenges
AU - Rauti, Rossana
AU - Renous, Noa
AU - Maoz, Ben M.
N1 - Publisher Copyright:
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/12
Y1 - 2020/12
N2 - The brain's extracellular matrix (ECM), a 3-dimensional macromolecular network that supports cell growth and viability, has crucial roles in homeostasis and disease. Accurate recapitulation of the chemical, structural, and mechanical properties of the ECM in brain cell cultures is essential for the development of translatable in vitro models; however, thus far, this task has proven highly challenging. This review provides an in-depth discussion of this challenge, including an overview of the properties of the ECM that in vitro models should endeavour to capture, a survey of ECM analogues that are currently used for this purpose, and a discussion of the main hindrances to developing more effective ECM-like coating materials.
AB - The brain's extracellular matrix (ECM), a 3-dimensional macromolecular network that supports cell growth and viability, has crucial roles in homeostasis and disease. Accurate recapitulation of the chemical, structural, and mechanical properties of the ECM in brain cell cultures is essential for the development of translatable in vitro models; however, thus far, this task has proven highly challenging. This review provides an in-depth discussion of this challenge, including an overview of the properties of the ECM that in vitro models should endeavour to capture, a survey of ECM analogues that are currently used for this purpose, and a discussion of the main hindrances to developing more effective ECM-like coating materials.
KW - Biocompatible materials
KW - In vitro neuronal cultures
KW - brain extracellular matrix
KW - microenvironment
KW - perineuronal nets
UR - http://www.scopus.com/inward/record.url?scp=85068679933&partnerID=8YFLogxK
U2 - 10.1002/ijch.201900052
DO - 10.1002/ijch.201900052
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AN - SCOPUS:85068679933
SN - 0021-2148
VL - 60
SP - 1141
EP - 1151
JO - Israel Journal of Chemistry
JF - Israel Journal of Chemistry
IS - 12
ER -