MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells

Lihi Ninio-Many; Hadas Grossman; Mattan Levi; Sofia Zilber; Ilan Tsarfaty; Noam Shomron; Anna Tuvar; Dana Chuderland; Salomon M. Stemmer; Irit Ben-Aharon; Ruth Shalgi

doi:10.18632/oncoscience.29

MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells

Lihi Ninio-Many, Hadas Grossman, Mattan Levi, Sofia Zilber, Ilan Tsarfaty, Noam Shomron, Anna Tuvar, Dana Chuderland, Salomon M. Stemmer, Irit Ben-Aharon^*, Ruth Shalgi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Fyn kinase is implicated in prostate cancer. We illustrate the role of miR-125a- 3p in cellular pathways accounted for motility and migration of prostate cancer cells, probably through its regulation on Fyn expression and Fyn-downstream proteins. Prostate cancer PC3 cells were transiently transfected with empty miR-Vec (control) or with miR-125a-3p. Overexpression of miR-125a-3p reduced migration of PC3 cells and increased apoptosis. Live cell confocal imaging indicated that overexpression of miR-125a-3p reduced the cells' track speed and length and impaired phenotype. Fyn, FAK and paxillin, displayed reduced activity following miR-125a-3p overexpression. Accordingly, actin rearrangement and cells' protrusion formation were impaired. An inverse correlation between miR-125a-3p and Gleason score was observed in human prostate cancer tissues. Our study demonstrated that miR-125a-3p may regulate migration of prostate cancer cells.

Original language	English
Pages (from-to)	250-261
Number of pages	12
Journal	Oncoscience
Volume	1
Issue number	4
DOIs	https://doi.org/10.18632/oncoscience.29
State	Published - 2014

Keywords

Actin cytoskeleton
EMT
Fyn
Live imaging
MiR-125a-3p
Migration
Prostate cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.18632/oncoscience.29

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@article{d011bd33d75241a0819731743eb5e2a7,

title = "MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells",

abstract = "Fyn kinase is implicated in prostate cancer. We illustrate the role of miR-125a- 3p in cellular pathways accounted for motility and migration of prostate cancer cells, probably through its regulation on Fyn expression and Fyn-downstream proteins. Prostate cancer PC3 cells were transiently transfected with empty miR-Vec (control) or with miR-125a-3p. Overexpression of miR-125a-3p reduced migration of PC3 cells and increased apoptosis. Live cell confocal imaging indicated that overexpression of miR-125a-3p reduced the cells' track speed and length and impaired phenotype. Fyn, FAK and paxillin, displayed reduced activity following miR-125a-3p overexpression. Accordingly, actin rearrangement and cells' protrusion formation were impaired. An inverse correlation between miR-125a-3p and Gleason score was observed in human prostate cancer tissues. Our study demonstrated that miR-125a-3p may regulate migration of prostate cancer cells.",

keywords = "Actin cytoskeleton, EMT, Fyn, Live imaging, MiR-125a-3p, Migration, Prostate cancer",

author = "Lihi Ninio-Many and Hadas Grossman and Mattan Levi and Sofia Zilber and Ilan Tsarfaty and Noam Shomron and Anna Tuvar and Dana Chuderland and Stemmer, {Salomon M.} and Irit Ben-Aharon and Ruth Shalgi",

note = "Funding Information: This work was supported by a grant from the Israel Science Foundation [grant number 261/09 to R.S.].",

year = "2014",

doi = "10.18632/oncoscience.29",

language = "אנגלית",

volume = "1",

pages = "250--261",

journal = "Oncoscience",

issn = "2331-4737",

publisher = "Impact Journals",

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TY - JOUR

T1 - MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells

AU - Ninio-Many, Lihi

AU - Grossman, Hadas

AU - Levi, Mattan

AU - Zilber, Sofia

AU - Tsarfaty, Ilan

AU - Shomron, Noam

AU - Tuvar, Anna

AU - Chuderland, Dana

AU - Stemmer, Salomon M.

AU - Ben-Aharon, Irit

AU - Shalgi, Ruth

N1 - Funding Information: This work was supported by a grant from the Israel Science Foundation [grant number 261/09 to R.S.].

PY - 2014

Y1 - 2014

N2 - Fyn kinase is implicated in prostate cancer. We illustrate the role of miR-125a- 3p in cellular pathways accounted for motility and migration of prostate cancer cells, probably through its regulation on Fyn expression and Fyn-downstream proteins. Prostate cancer PC3 cells were transiently transfected with empty miR-Vec (control) or with miR-125a-3p. Overexpression of miR-125a-3p reduced migration of PC3 cells and increased apoptosis. Live cell confocal imaging indicated that overexpression of miR-125a-3p reduced the cells' track speed and length and impaired phenotype. Fyn, FAK and paxillin, displayed reduced activity following miR-125a-3p overexpression. Accordingly, actin rearrangement and cells' protrusion formation were impaired. An inverse correlation between miR-125a-3p and Gleason score was observed in human prostate cancer tissues. Our study demonstrated that miR-125a-3p may regulate migration of prostate cancer cells.

AB - Fyn kinase is implicated in prostate cancer. We illustrate the role of miR-125a- 3p in cellular pathways accounted for motility and migration of prostate cancer cells, probably through its regulation on Fyn expression and Fyn-downstream proteins. Prostate cancer PC3 cells were transiently transfected with empty miR-Vec (control) or with miR-125a-3p. Overexpression of miR-125a-3p reduced migration of PC3 cells and increased apoptosis. Live cell confocal imaging indicated that overexpression of miR-125a-3p reduced the cells' track speed and length and impaired phenotype. Fyn, FAK and paxillin, displayed reduced activity following miR-125a-3p overexpression. Accordingly, actin rearrangement and cells' protrusion formation were impaired. An inverse correlation between miR-125a-3p and Gleason score was observed in human prostate cancer tissues. Our study demonstrated that miR-125a-3p may regulate migration of prostate cancer cells.

KW - Actin cytoskeleton

KW - EMT

KW - Fyn

KW - Live imaging

KW - MiR-125a-3p

KW - Migration

KW - Prostate cancer

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AN - SCOPUS:84921405487

SN - 2331-4737

VL - 1

SP - 250

EP - 261

JO - Oncoscience

JF - Oncoscience

IS - 4

ER -

MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells

Abstract

Keywords

UN SDGs

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