MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells

Lihi Ninio-Many, Hadas Grossman, Mattan Levi, Sofia Zilber, Ilan Tsarfaty, Noam Shomron, Anna Tuvar, Dana Chuderland, Salomon M. Stemmer, Irit Ben-Aharon*, Ruth Shalgi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Fyn kinase is implicated in prostate cancer. We illustrate the role of miR-125a- 3p in cellular pathways accounted for motility and migration of prostate cancer cells, probably through its regulation on Fyn expression and Fyn-downstream proteins. Prostate cancer PC3 cells were transiently transfected with empty miR-Vec (control) or with miR-125a-3p. Overexpression of miR-125a-3p reduced migration of PC3 cells and increased apoptosis. Live cell confocal imaging indicated that overexpression of miR-125a-3p reduced the cells' track speed and length and impaired phenotype. Fyn, FAK and paxillin, displayed reduced activity following miR-125a-3p overexpression. Accordingly, actin rearrangement and cells' protrusion formation were impaired. An inverse correlation between miR-125a-3p and Gleason score was observed in human prostate cancer tissues. Our study demonstrated that miR-125a-3p may regulate migration of prostate cancer cells.

Original languageEnglish
Pages (from-to)250-261
Number of pages12
JournalOncoscience
Volume1
Issue number4
DOIs
StatePublished - 2014

Funding

FundersFunder number
Israel Science Foundation261/09

    Keywords

    • Actin cytoskeleton
    • EMT
    • Fyn
    • Live imaging
    • MiR-125a-3p
    • Migration
    • Prostate cancer

    Fingerprint

    Dive into the research topics of 'MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells'. Together they form a unique fingerprint.

    Cite this