Microfluidic Neuromuscular Co-culture System for Tracking Cell-to-Cell Transfer and Axonal Transport of Labeled Proteins

Ariel Ionescu, Eran Perlson*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations

Abstract

The molecular communication mechanisms within the Motor Neurons (MN) distant axon and its soma, as well as between MN and their neighboring cells and extracellular environment are of keen interest for our understanding of neurodevelopment and neurodegenerative diseases. One tool that has significantly improved our ability to study such processes with high spatiotemporal resolution is microfluidic devices. Here we describe a step-by-step guide to the neuromuscular co-culturing procedure and demonstrate how to track trophic factors transmission from muscle-to-neuron and their transport along the axons.

Original languageEnglish
Title of host publicationAxonal transport
EditorsAlessio Vagnoni
PublisherHumana Press Inc.
Pages145-161
Number of pages17
ISBN (Electronic)9781071619902
ISBN (Print)9781071619896
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2431
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Axonal transport
  • BDNF
  • GDNF
  • Microfluidic chambers
  • Motor neurons
  • Neuromuscular junction
  • Skeletal muscles

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