TY - GEN
T1 - Microbuckling of fibrous matrices enables long range cell mechanosensing
AU - Burkel, Brian
AU - Lesman, Ayelet
AU - Rosakis, Phoebus
AU - Tirrell, David A.
AU - Ravichandran, Guruswami
AU - Notbohm, Jacob
N1 - Publisher Copyright:
© The Society for Experimental Mechanics, Inc. 2017.
PY - 2017
Y1 - 2017
N2 - When biological cells migrate, divide, and invade, they push and pull on individual fibers of the matrix surrounding them. The resulting fiber displacements are neither uniform nor smooth; rather, displacements localize to form dense fibrous bands that span from one cell to another. It is thought that these bands may be a mechanism by which cells can sense their neighbors, but this hypothesis remains untested, because the mechanism for band formation remains unknown. Using digital volume correlation, we measure the displacements induced by contractile cells embedded in a fibrous matrix. We find that cell-induced displacements propagate over a longer range than predicted by linear elasticity. To explain the long-range propagation of displacements, we consider the effect of buckling of individual matrix fibers, which generates a nonlinear stress-strain relationship. We show that fiber buckling is the mechanism that causes the displacements to propagate over a long range and the bands to form between nearby cells. The results thus show that buckling of individual fibers provides a mechanism by which cells may sense their distant neighbors mechanically.
AB - When biological cells migrate, divide, and invade, they push and pull on individual fibers of the matrix surrounding them. The resulting fiber displacements are neither uniform nor smooth; rather, displacements localize to form dense fibrous bands that span from one cell to another. It is thought that these bands may be a mechanism by which cells can sense their neighbors, but this hypothesis remains untested, because the mechanism for band formation remains unknown. Using digital volume correlation, we measure the displacements induced by contractile cells embedded in a fibrous matrix. We find that cell-induced displacements propagate over a longer range than predicted by linear elasticity. To explain the long-range propagation of displacements, we consider the effect of buckling of individual matrix fibers, which generates a nonlinear stress-strain relationship. We show that fiber buckling is the mechanism that causes the displacements to propagate over a long range and the bands to form between nearby cells. The results thus show that buckling of individual fibers provides a mechanism by which cells may sense their distant neighbors mechanically.
KW - DVC
KW - Extracellular matrix
KW - Fiber buckling
KW - Mechanosensing
KW - Traction force microscopy
UR - http://www.scopus.com/inward/record.url?scp=84990057860&partnerID=8YFLogxK
U2 - 10.1007/978-3-319-41351-8_19
DO - 10.1007/978-3-319-41351-8_19
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AN - SCOPUS:84990057860
SN - 9783319413501
T3 - Conference Proceedings of the Society for Experimental Mechanics Series
SP - 135
EP - 141
BT - Mechanics of Biological Systems and Materials - Proceedings of the 2016 Annual Conference on Experimental and Applied Mechanics
A2 - Korach, Chad S.
A2 - Tekalur, Srinivasan Arjun
A2 - Zavattieri, Pablo
PB - Springer New York LLC
T2 - Annual Conference and Exposition on Experimental and Applied Mechanics, 2016
Y2 - 6 June 2016 through 9 June 2016
ER -