Abstract
This communication describes the methods for the purification of the elongation factor G from B. subtilis cells and the characterization of its activity by a direct means using 3H labeled puromycin and 'natural' peptidyl tRNA molecules. The application of this method to the analysis of a temperature sensitive mutant blocked in protein synthesis is discussed.
| Original language | English |
|---|---|
| Title of host publication | AMER.SOC.MICROBIOL.,WASHINGTON |
| Pages | 194-201 |
| Number of pages | 8 |
| State | Published - 1976 |