This communication describes the methods for the purification of the elongation factor G from B. subtilis cells and the characterization of its activity by a direct means using 3H labeled puromycin and 'natural' peptidyl tRNA molecules. The application of this method to the analysis of a temperature sensitive mutant blocked in protein synthesis is discussed.
|Title of host publication||AMER.SOC.MICROBIOL.,WASHINGTON|
|Number of pages||8|
|State||Published - 1976|