Microbial alcohol-conferred hemolysis is a late response to alcohol stress

Amir Shuster, Moshe Korem, Jasmine Jacob-Hirsch, Ninette Amariglio, Gideon Rechavi, Mel Rosenberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We have reported previously that growth on alcohol vapors confers hemolytic properties on certain yeast species and strains ['microbial alcohol-conferred hemolysis' (MACH)]. In a recent study, we analyzed the genetic basis of MACH in Saccharomyces cerevisiae using the EUROSCARF mutant collection. The data suggested that intact mitochondrial and respiratory chain functions are critical for the observed alcohol-mediated hemolysis. We proposed that the uncontrolled cellular uptake of alcohol results in yeast 'hyper-respiration', leading to elaboration of hemolytic molecules such as hydrogen peroxide and lytic lipids. In the current study, we have further analyzed the molecular mechanisms involved in the MACH phenomenon in S. cerevisiae, using DNA microarrays. The patterns of regulation were confirmed by quantitative reverse transcriptase PCR. The results presented here lend further support to this hypothesis, based on upregulation of the genes responsible for coping with vast amounts of hydrogen peroxide produced as a byproduct of excessive oxidation of alcohol. These results, taken together, show that alcohol-mediated hemolysis in yeast appears to be related to the overproduction of hemolytic byproducts, particularly hydrogen peroxide, which accumulates during long-term exposure of S. cerevisiae to both ethanol and n-butanol.

Original languageEnglish
Pages (from-to)315-323
Number of pages9
JournalFEMS Yeast Research
Volume11
Issue number4
DOIs
StatePublished - Jun 2011

Keywords

  • Ethanol
  • Hemolysis
  • Hydrogen peroxide
  • Respiration
  • Yeast
  • n-butanol

Fingerprint

Dive into the research topics of 'Microbial alcohol-conferred hemolysis is a late response to alcohol stress'. Together they form a unique fingerprint.

Cite this