Microassay of thiamine and its phosphate esters after separation by paper chromatography

Lawrence M. Lewin, Robert Wei

Research output: Contribution to journalArticlepeer-review

Abstract

Thiamine and its mono- and pyrophosphate esters (0-1 μg) were separated by paper chromatography, converted to their respective thiochromes, and measured fluorometrically. Excellent recoveries of added thiamine and thiamine phosphate from protein-containing mixtures were achieved by HCl extraction.

Original languageEnglish
Pages (from-to)29-35
Number of pages7
JournalAnalytical Biochemistry
Volume16
Issue number1
DOIs
StatePublished - Jul 1966

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