TY - JOUR
T1 - Microassay of thiamine and its phosphate esters after separation by paper chromatography
AU - Lewin, Lawrence M.
AU - Wei, Robert
N1 - Funding Information:
This study was supported in part by research grant AM-0839302 of the U. S. National Institutes of Health. The authors wish to acknowledge the helpful suggestions presented by Dr. Mortimer Lorber.
PY - 1966/7
Y1 - 1966/7
N2 - Thiamine and its mono- and pyrophosphate esters (0-1 μg) were separated by paper chromatography, converted to their respective thiochromes, and measured fluorometrically. Excellent recoveries of added thiamine and thiamine phosphate from protein-containing mixtures were achieved by HCl extraction.
AB - Thiamine and its mono- and pyrophosphate esters (0-1 μg) were separated by paper chromatography, converted to their respective thiochromes, and measured fluorometrically. Excellent recoveries of added thiamine and thiamine phosphate from protein-containing mixtures were achieved by HCl extraction.
UR - http://www.scopus.com/inward/record.url?scp=0013924969&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(66)90077-7
DO - 10.1016/0003-2697(66)90077-7
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AN - SCOPUS:0013924969
SN - 0003-2697
VL - 16
SP - 29
EP - 35
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -