Methylene blue prevents pulmonary injury after intestinal ischemia- reperfusion

Yair Galili, Ron Ben-Abraham, Abraham Weinbroum, Silvia Marmur, Adrian Iaina, Yoram Volman, Gari Peer, Oded Szold, Dror Soffer, Josef Klausner, Micha Rabau, Yoram Kluger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Objective: To investigate the effect of methylene blue, an inhibitor of oxygen radicals, on lung injury caused by reperfusion of ischemic tissue. Methods: Intestinal ischemia-reperfusion injury was induced in rats by clamping the superior mesenteric artery for 1 hour. Thereafter, the experimental group was administered 1% methylene blue intraperitoneally and the control group received saline. After 4 hours, pulmonary histopathologic features were assessed, and lung wet-weight to dry-weight ratios and tissue xanthine oxidase were determined. Results: The control group suffered from severe pulmonary parenchymal damage, compared with slight damage in the experimental group. The number of sequestered neutrophils was significantly higher in the control group (319 ± 60 polymorphonuclear cells per 10 high- power fields) than in the methylene blue-treated group (91 ± 8 polymorphonuclear cells per 10 high-power fields; p < 0.001). The wet-weight to dry-weight ratio was significantly increased in the saline-treated rats compared with the methylene blue-treated group (6.19 ± 0.28 vs. 5.07 ± 0.21; p < 0.001). Xanthine oxidase activity was similar in both groups. Conclusion: Methylene blue attenuated lung injury after intestinal ischemia- reperfusion. Inhibition of oxygen free radicals may be the protective mechanism.

Original languageEnglish
Pages (from-to)222-226
Number of pages5
JournalJournal of Trauma
Issue number2
StatePublished - Aug 1998


  • Acute lung injury
  • Methylene blue
  • Oxygen free radicals


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