Methylated simian virus 40 specific RNA from nuclei and cytoplasm of infected BSC 1 cells

S. Lavi, A. J. Shatkin

Research output: Contribution to journalArticlepeer-review


Host cell and virus specific poly(A) containing RNAs isolated from nuclei and cytoplasm of monkey kidney cells infected with simian virus 40 contain different methylated nucleotides. In the cytoplasmic simian virus 40 specific RNA, about 75% of the radioactivity derived from [methyl 3H]methionine was in N6 methyladenosine (N6mA) after digestion with Penicillium nuclease and bacterial alkaline phosphatase. The remainder was in a negatively charged component with properties of 5' terminal structures, i.e., digestion with nucleotide pyrophosphatase and bacterial alkaline phosphatase released 2' O methyladenosine (A[m]), 2' O methylguanosine (G[m]), and 7 methylguanosine (m7G), consistent with a 5' terminal structure of the type, m7GpppN(m). The nuclear virus specific RNA contained N6mA, G(m), 2' O methyluridine (U[m]), and a smaller proportion (10%) of nuclease, phosphatase resistant presumptive 5' termini that also yielded A(m), G(m), and m7G upon further hydrolysis. The infected cell nuclear and cytoplasmic RNAs that did not hybridize to DNA of simian virus 40 contained all four 2' O methylnucleosides. The possible role of methylation in the processing and translation of simian virus 40 specific mRNA is discussed.

Original languageEnglish
Pages (from-to)2012-2016
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number6
StatePublished - 1975
Externally publishedYes


Dive into the research topics of 'Methylated simian virus 40 specific RNA from nuclei and cytoplasm of infected BSC 1 cells'. Together they form a unique fingerprint.

Cite this