Host cell and virus specific poly(A) containing RNAs isolated from nuclei and cytoplasm of monkey kidney cells infected with simian virus 40 contain different methylated nucleotides. In the cytoplasmic simian virus 40 specific RNA, about 75% of the radioactivity derived from [methyl 3H]methionine was in N6 methyladenosine (N6mA) after digestion with Penicillium nuclease and bacterial alkaline phosphatase. The remainder was in a negatively charged component with properties of 5' terminal structures, i.e., digestion with nucleotide pyrophosphatase and bacterial alkaline phosphatase released 2' O methyladenosine (A[m]), 2' O methylguanosine (G[m]), and 7 methylguanosine (m7G), consistent with a 5' terminal structure of the type, m7GpppN(m). The nuclear virus specific RNA contained N6mA, G(m), 2' O methyluridine (U[m]), and a smaller proportion (10%) of nuclease, phosphatase resistant presumptive 5' termini that also yielded A(m), G(m), and m7G upon further hydrolysis. The infected cell nuclear and cytoplasmic RNAs that did not hybridize to DNA of simian virus 40 contained all four 2' O methylnucleosides. The possible role of methylation in the processing and translation of simian virus 40 specific mRNA is discussed.
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - 1975|