TY - JOUR
T1 - Methyl jasmonate reduces the survival of cervical cancer cells and downregulates HPV E6 and E7, and survivin
AU - Milrot, Elad
AU - Jackman, Anna
AU - Kniazhanski, Tatiana
AU - Gonen, Pinhas
AU - Flescher, Eliezer
AU - Sherman, Levana
N1 - Funding Information:
We are grateful to Dr. Ilan Tsarfaty, Department of Human Microbiology at the Sackler School of Medicine for his assistance in tissue staining image analysis. This research was supported by a research Grant of the Chief Scientist’s Office, Ministry of Health, Israel, awarded to LS and in part by a Grant from the Bernard Jacobson Fund for Cancer Research, Tel-Aviv University awarded to LS.
PY - 2012/6/1
Y1 - 2012/6/1
N2 - The present study further investigated the mode of action of methyl jasmonate (MJ) in different cervical cancer cell lines. We show that in addition to the short term cytotoxicity, MJ effectively reduced the survival of cervical cancer cells (clonogenicity assays). MJ induced apoptosis in all cervical cancer cells. In some cell lines, MJ caused elevation of the mitochondrial superoxide anion, notably, in HeLa and CaSki. Changes in the expression of p53 and bax were variable, yet, downregulation of survivin was common to all cervical cancer cells. MJ significantly reduced the levels of the human papillomavirus (HPV) E6 and E7 proteins without alteration of the mRNA levels. Moreover, ectopic expression of E6, E7 or both in cervical cancer cells that lack HPV (C33A), did not alter significantly their response to MJ. Our studies point to MJ as an effective anticancer agent against a variety of cervical cancer cells acting through shared and different pathways to induce cell death regardless of the presence of HPV.
AB - The present study further investigated the mode of action of methyl jasmonate (MJ) in different cervical cancer cell lines. We show that in addition to the short term cytotoxicity, MJ effectively reduced the survival of cervical cancer cells (clonogenicity assays). MJ induced apoptosis in all cervical cancer cells. In some cell lines, MJ caused elevation of the mitochondrial superoxide anion, notably, in HeLa and CaSki. Changes in the expression of p53 and bax were variable, yet, downregulation of survivin was common to all cervical cancer cells. MJ significantly reduced the levels of the human papillomavirus (HPV) E6 and E7 proteins without alteration of the mRNA levels. Moreover, ectopic expression of E6, E7 or both in cervical cancer cells that lack HPV (C33A), did not alter significantly their response to MJ. Our studies point to MJ as an effective anticancer agent against a variety of cervical cancer cells acting through shared and different pathways to induce cell death regardless of the presence of HPV.
KW - Apoptosis
KW - Cervical cancer
KW - HPV E6 and E7
KW - Mitochondrial superoxide
KW - Survivin
UR - http://www.scopus.com/inward/record.url?scp=84858071650&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2011.12.028
DO - 10.1016/j.canlet.2011.12.028
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AN - SCOPUS:84858071650
SN - 0304-3835
VL - 319
SP - 31
EP - 38
JO - Cancer Letters
JF - Cancer Letters
IS - 1
ER -
