Methotrexate inhibits proliferation but not interleukin 1 stimulated secretory activities of cultured human synovial fibroblasts

F. A. Meyer, I. Yaron, V. Mashiah, M. Yaron*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

The effect of methotrexate (MTX) on proliferation and on interleukin 1 stimulated secretory activities of human synovial fibroblasts in culture was investigated. MTX caused a dose dependent inhibition of growth over the concentration range 0.07-2.2 μM with a half-maximal effect at 0.37 μM. Inhibition was competitively relieved by coaddition of leucovorin. Cell growth was fully restored after MTX pretreatment of 24 h but not after 48 h, even on subsequent leucovorin addition. Cell viability was unaffected by MTX treatment. MTX had no effect on interleukin 1 stimulated production of prostaglandin E, hyaluronic acid and collagenase. Our results raise the possibility that one of the mechanisms contributing to the therapeutic effects of MTX in patients with rheumatoid arthritis may involve modulation of synovial fibroblast growth.

Original languageEnglish
Pages (from-to)238-242
Number of pages5
JournalJournal of Rheumatology
Volume20
Issue number2
StatePublished - 1993
Externally publishedYes

Keywords

  • cell proliferation
  • methotrexate
  • rheumatoid arthritis
  • synovial fibroblasts

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