TY - JOUR
T1 - Method for embedding temporal bones of rats in methyl-methacrylate
AU - Nageris, Benny
AU - Gazit, Dan
N1 - Funding Information:
Nageris Benny MD Worcester, Massachusetts Gazit Dan PhD Jerusalem, Israel Reprints — Benny Nageris, MD, Dept of Otolaryngology-Head and Neck Surgery, University of Massachusetts Medical Center, 55 Lake Ave N, Worcester, MA 01655 From the Department of Otolaryngology-Head and Neck Surgery, University of Massachusetts Medical Center, Worcester, Massachusetts (Nageris), and the Bone Laboratory, Hebrew University-Hadassah, Faculty of Dental Medicine, Jerusalem, Israel (Gazit). This study was performed in accordance with the PHS Policy on Humane Care and Use of Laboratory Animals, the NIH Guide for the Care and Use of Laboratory Animals , and the Animal Welfare Act (7 U.S.C. et seq.); the animal use protocol was approved by the Institutional Animal Care and Use Committee (IACUC) of Hebrew University-Hadassah, Jerusalem, Israel. 10 1995 104 10 783 785 © 1995 SAGE Publications 1995 SAGE Publications The commonly used method of preparing the temporal bone for light microscopy is a refinement of a basic formula that has been employed for a century. This process includes fixation, decalcification, neutralization, dehydration, embedding in celloidin, and hardening. The main disadvantage of this process is that decalcification is performed. This article describes a new method for preparing the temporal bone of rats for light microscopy. The main advantage of this new method is that no decalcification is involved, so that all bony elements are retained in their normal shape and location, and even retain some enzymatic activity. Other advantages are that the fixation is reversible and the process is short (approximately 2 weeks) and therefore relatively inexpensive. Our vast and positive experience with this technique has led us to report this method not in a specific experiment, but rather as a specific laboratory technique. embedding histopathology methyl-methacrylate temporal bone.
PY - 1995/10
Y1 - 1995/10
N2 - The commonly used method of preparing the temporal bone for light microscopy is a refinement of a basic formula that has been employed for a century. This process includes fixation, decalcification, neutralization, dehydration, embedding in celloidin, and hardening. The main disadvantage of this process is that decalcification is performed. This article describes a new method for preparing the temporal bone of rats for light microscopy. The main advantage of this new method is that no decalcification is involved, so that all bony elements are retained in their normal shape and location, and even retain some enzymatic activity. Other advantages are that the fixation is reversible and the process is short (approximately 2 weeks) and therefore relatively inexpensive. Our vast and positive experience with this technique has led us to report this method not in a specific experiment, but rather as a specific laboratory technique.
AB - The commonly used method of preparing the temporal bone for light microscopy is a refinement of a basic formula that has been employed for a century. This process includes fixation, decalcification, neutralization, dehydration, embedding in celloidin, and hardening. The main disadvantage of this process is that decalcification is performed. This article describes a new method for preparing the temporal bone of rats for light microscopy. The main advantage of this new method is that no decalcification is involved, so that all bony elements are retained in their normal shape and location, and even retain some enzymatic activity. Other advantages are that the fixation is reversible and the process is short (approximately 2 weeks) and therefore relatively inexpensive. Our vast and positive experience with this technique has led us to report this method not in a specific experiment, but rather as a specific laboratory technique.
KW - embedding
KW - histopathology
KW - methyl-methacrylate
KW - temporal bone
UR - http://www.scopus.com/inward/record.url?scp=0028845696&partnerID=8YFLogxK
U2 - 10.1177/000348949510401006
DO - 10.1177/000348949510401006
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C2 - 7574255
AN - SCOPUS:0028845696
SN - 0003-4894
VL - 104
SP - 783
EP - 785
JO - Annals of Otology, Rhinology and Laryngology
JF - Annals of Otology, Rhinology and Laryngology
IS - 10
ER -