Methionine biosynthesis in Agrobacterium tumefaciens: Study of the first enzyme

Or Rotem, Dvora Biran, Eliora Z. Ron

Research output: Contribution to journalArticlepeer-review

Abstract

Here we characterize the first step in methionine biosynthesis in Agrobacterium tumefaciens, an α-proteobacterium. We explored the metA gene and its products and found several unique properties. Although the gene was annotated as a homoserine transsuccinylase, based upon sequence similarity to characterized homologs in other bacteria, including Escherichia coli, the enzyme uses acetyl-CoA as a substrate and therefore is functionally a transacetylase. Moreover, the protein is thermolabile and the gene is under regulation of heat shock transcriptional activator σ32. 3. The gene has a SAM-riboswitch, which shuts off transcription by σ-32 as well as by the vegetative σ-70.

Original languageEnglish
Pages (from-to)12-16
Number of pages5
JournalResearch in Microbiology
Volume164
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • Agrobacterium tumefaciens
  • Heat shock gene
  • Homoserine acetyl-transferase
  • Homoserine trans-succinylase
  • Methionine biosynthesis
  • Riboswitch

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