TY - JOUR
T1 - Metal nanoparticles released from dental implant surfaces
T2 - Potential contribution to chronic inflammation and peri-implant bone loss
AU - Bressan, Eriberto
AU - Ferroni, Letizia
AU - Gardin, Chiara
AU - Bellin, Gloria
AU - Sbricoli, Luca
AU - Sivolella, Stefano
AU - Brunello, Giulia
AU - Schwartz-Arad, Devorah
AU - Mijiritsky, Eitan
AU - Penarrocha, Miguel
AU - Penarrocha, David
AU - Taccioli, Cristian
AU - Tatullo, Marco
AU - Piattelli, Adriano
AU - Zavan, Barbara
N1 - Publisher Copyright:
© 2019 by the authors.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Peri-implantitis is an inflammatory disease affecting tissues surrounding dental implants. Although it represents a common complication of dental implant treatments, the underlying mechanisms have not yet been fully described. The aim of this study is to identify the role of titanium nanoparticles released form the implants on the chronic inflammation and bone lysis in the surrounding tissue. We analyzed the in vitro effect of titanium (Ti) particle exposure on mesenchymal stem cells (MSCs) and fibroblasts (FU), evaluating cell proliferation by MTT test and the generation of reactive oxygen species (ROS). Subsequently, in vivo analysis of peri-implant Ti particle distribution, histological, and molecular analyses were performed. Ti particles led to a time-dependent decrease in cell viability and increase in ROS production in both MSCs and FU. Tissue analyses revealed presence of oxidative stress, high extracellular and intracellular Ti levels and imbalanced bone turnover. High expression of ZFP467 and the presence of adipose-like tissue suggested dysregulation of the MSC population; alterations in vessel morphology were identified. The results suggest that Ti particles may induce the production of high ROS levels, recruiting abnormal quantity of neutrophils able to produce high level of metalloproteinase. This induces the degradation of collagen fibers. These events may influence MSC commitment, with an imbalance of bone regeneration.
AB - Peri-implantitis is an inflammatory disease affecting tissues surrounding dental implants. Although it represents a common complication of dental implant treatments, the underlying mechanisms have not yet been fully described. The aim of this study is to identify the role of titanium nanoparticles released form the implants on the chronic inflammation and bone lysis in the surrounding tissue. We analyzed the in vitro effect of titanium (Ti) particle exposure on mesenchymal stem cells (MSCs) and fibroblasts (FU), evaluating cell proliferation by MTT test and the generation of reactive oxygen species (ROS). Subsequently, in vivo analysis of peri-implant Ti particle distribution, histological, and molecular analyses were performed. Ti particles led to a time-dependent decrease in cell viability and increase in ROS production in both MSCs and FU. Tissue analyses revealed presence of oxidative stress, high extracellular and intracellular Ti levels and imbalanced bone turnover. High expression of ZFP467 and the presence of adipose-like tissue suggested dysregulation of the MSC population; alterations in vessel morphology were identified. The results suggest that Ti particles may induce the production of high ROS levels, recruiting abnormal quantity of neutrophils able to produce high level of metalloproteinase. This induces the degradation of collagen fibers. These events may influence MSC commitment, with an imbalance of bone regeneration.
KW - Osteolysis
KW - Reactive oxygen species
KW - Titanium nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85067924206&partnerID=8YFLogxK
U2 - 10.3390/ma12122036
DO - 10.3390/ma12122036
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:85067924206
SN - 1996-1944
VL - 12
JO - Materials
JF - Materials
IS - 12
M1 - 2036
ER -