Metabolomic Analysis of Natural Variation in Arabidopsis

Si Wu, Saleh Alseekh, Yariv Brotman, Alisdair R. Fernie*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Methodological advances in coupled-mass spectrometry (gas chromatography and liquid chromatography; GC-MS and LC-MS) have rendered the profiling of highly complex plant extracts relatively facile and allowed that their high-throughput use aids the investigation of a range of biological questions. Among these is the elucidation of the genetic factors underlying metabolite abundance. For this purpose genome-wide association studies (GWAS) are being widely adopted in Arabidopsis with the resultant quantitative trait loci being subjected to cross-validation by the use of recombinant inbred lines, introgression lines, and T-DNA insertional knockout lines.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages393-411
Number of pages19
DOIs
StatePublished - 2021
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2200
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Funding

FundersFunder number
Horizon 2020 Framework Programme739582, 664621, 664620

    Keywords

    • Gas-chromatography mass-spectrometry
    • Genome-wide association mapping
    • Liquid-chromatography mass-spectrometry
    • Metabolomics
    • QTL analysis

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