TY - JOUR
T1 - Metabolites from microcystis aeruginosa bloom material collected at a water reservoir near Kibbutz Hafetz Haim, Israel
AU - Lodin-Friedman, Anat
AU - Carmeli, Shmuel
PY - 2013/6/28
Y1 - 2013/6/28
N2 - An aqueous MeOH extract of Microcystis aeruginosa (IL-399) afforded three new protease inhibitors, micropeptin HH978 (1), micropeptin HH960 (2), and micropeptin HH992 (3), as well as the known aeruginosin GH553 and microguanidine AL772. The structures of the compounds were elucidated using 1D and 2D NMR techniques, as well as high-resolution mass spectrometry. The absolute configurations of 1-3 were determined using Marfey's method for amino acid and chiral-phase HPLC for hydroxy acids. The inhibitory activity of the compounds was determined for the serine proteases trypsin, thrombin, elastase, and chymotrypsin. The structure elucidation and biological activities of the new natural products are discussed.
AB - An aqueous MeOH extract of Microcystis aeruginosa (IL-399) afforded three new protease inhibitors, micropeptin HH978 (1), micropeptin HH960 (2), and micropeptin HH992 (3), as well as the known aeruginosin GH553 and microguanidine AL772. The structures of the compounds were elucidated using 1D and 2D NMR techniques, as well as high-resolution mass spectrometry. The absolute configurations of 1-3 were determined using Marfey's method for amino acid and chiral-phase HPLC for hydroxy acids. The inhibitory activity of the compounds was determined for the serine proteases trypsin, thrombin, elastase, and chymotrypsin. The structure elucidation and biological activities of the new natural products are discussed.
UR - http://www.scopus.com/inward/record.url?scp=84879664330&partnerID=8YFLogxK
U2 - 10.1021/np400281q
DO - 10.1021/np400281q
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AN - SCOPUS:84879664330
SN - 0163-3864
VL - 76
SP - 1196
EP - 1200
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 6
ER -