TY - JOUR
T1 - Membrane fusion induced by the membrane mobility agent, A2C Differentiation between fusible and non-fusible cells Transfer of fusibility
AU - Tavassoli, Mehdi
AU - Kosower, Nechama S.
AU - Halverson, Craig
AU - Aoki, Makoto
AU - Kosower, Edward M.
N1 - Funding Information:
This work was supported by NIH grants AM 25510, AM 70551 and DOE-EV00899 to M.T.N.S.K. has been the recipient of a United States-Israel Binational Science Foundation (BSF) grant. E.M.K. was a J.S. Guggenheim Fellow, University of California, San Diego and University of California, Berkeley 1977--1978. M.T. is a recipient of Research Career Development Award for National Institute of Arthritis, Rheumatism and Digestive Disease.
PY - 1980
Y1 - 1980
N2 - Red cells of different species respond differently to the treatment with the membrane mobility agent, A2C, with respect to both the A2C interaction and the subsequent cell-cell interaction. Depending on whether both, one or neither of the processes are effective, some red cells (e.g., nucleated Leghorn hen red cells, rat red cells) fuse easily, some (human red cells) show morphological changes but do not fuse, and others (nucleated Rock hen red cells) show little or no response. Mixed fusion (i.e., between fusible cells of different species) is readily obtained, indicating that no species-specific recognition sites are required for A2C-induced fusion. The potential for fusion is a transferable characteristic. In the presence of fusible cells, A2C induces both heterologous and homologous fusion of otherwise 'non-fusible' cells. Electron micrographs of fusing cells after treatment with A2C reveal 'onion-ring' structures ('whorls'), free of intramembranous protein particles but different from the smooth appearance of A2C particles. Whorls are considered to arise from fusion-potent membrane areas. Fusion is apparent at multiple sites along the contact line between apposed membranes. The postulated appearance of vesicle-like structures along the fusion line (Kosower, E.M., Kosower, N.S. and Wegman, P. (1977) Biochim. Biophys. Acta 471, 311-329) is confirmed by micrographs. The mechanism of this fusion process is discussed and compared to other types of fusion process.
AB - Red cells of different species respond differently to the treatment with the membrane mobility agent, A2C, with respect to both the A2C interaction and the subsequent cell-cell interaction. Depending on whether both, one or neither of the processes are effective, some red cells (e.g., nucleated Leghorn hen red cells, rat red cells) fuse easily, some (human red cells) show morphological changes but do not fuse, and others (nucleated Rock hen red cells) show little or no response. Mixed fusion (i.e., between fusible cells of different species) is readily obtained, indicating that no species-specific recognition sites are required for A2C-induced fusion. The potential for fusion is a transferable characteristic. In the presence of fusible cells, A2C induces both heterologous and homologous fusion of otherwise 'non-fusible' cells. Electron micrographs of fusing cells after treatment with A2C reveal 'onion-ring' structures ('whorls'), free of intramembranous protein particles but different from the smooth appearance of A2C particles. Whorls are considered to arise from fusion-potent membrane areas. Fusion is apparent at multiple sites along the contact line between apposed membranes. The postulated appearance of vesicle-like structures along the fusion line (Kosower, E.M., Kosower, N.S. and Wegman, P. (1977) Biochim. Biophys. Acta 471, 311-329) is confirmed by micrographs. The mechanism of this fusion process is discussed and compared to other types of fusion process.
KW - (Red cell)
KW - Membrane fusion
KW - Mobility agent AC
UR - http://www.scopus.com/inward/record.url?scp=0019133914&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(80)90557-X
DO - 10.1016/0005-2736(80)90557-X
M3 - מאמר
AN - SCOPUS:0019133914
VL - 601
SP - 544
EP - 558
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - C
ER -