Mechanochemical synthesis of nanocrystalline hydroxyapatite from Mercenaria clam shells and phosphoric acid

Anindya Pal; Shubhadeep Maity; Sumit Chabri; Supriya Bera; Amit Roy Chowdhury; Mitun Das; Arijit Sinha

doi:10.1088/2057-1976/aa54f5

Mechanochemical synthesis of nanocrystalline hydroxyapatite from Mercenaria clam shells and phosphoric acid

Anindya Pal, Shubhadeep Maity, Sumit Chabri, Supriya Bera, Amit Roy Chowdhury, Mitun Das^*, Arijit Sinha

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of the present study is to synthesize hydroxyapatite from seashell and to explore its biocompatibility in vitro. Nano-crystalline hydroxyapatite (HAp) ceramics were successfully fabricated by a mechanochemical method using clam seashells and phosphoric acid. The CaO and H₃PO₄ acid at different w.t% ratios i.e. 1:0.75, 1:1.25, 1:1.5 and 1:1.75, were ball milled and then heat treated at 1000 °C for 3 h to complete reactions. The synthesized powders were characterized using x-ray diffraction (XRD), FTIR spectroscopy, scanning electron microscope and high resolution transmission electron microscopy. XRD results showed that the average crystallite size of the powder varies from 53 to 67 nm and crystallinity of powder found to be in the range of 88% and 96%. In vitro biocompatibility studies were carried out using osteoblast (MG63) and fibroblast cells (NIH3T3), demonstrated non-toxic nature of the seashell derived HAp powder.

Original language	English
Article number	015010
Journal	Biomedical Physics and Engineering Express
Volume	3
Issue number	1
DOIs	https://doi.org/10.1088/2057-1976/aa54f5
State	Published - 13 Jan 2017
Externally published	Yes

Keywords

Biocompatibity
Hydroxyapatite
MTT assay
Mechanochemical synthesis
Sea shell
X-ray diffraction

Access to Document

10.1088/2057-1976/aa54f5

Cite this

@article{72a4d6811cbc4c4b9314e5f6c6e7ef9c,

title = "Mechanochemical synthesis of nanocrystalline hydroxyapatite from Mercenaria clam shells and phosphoric acid",

abstract = "The aim of the present study is to synthesize hydroxyapatite from seashell and to explore its biocompatibility in vitro. Nano-crystalline hydroxyapatite (HAp) ceramics were successfully fabricated by a mechanochemical method using clam seashells and phosphoric acid. The CaO and H3PO4 acid at different w.t% ratios i.e. 1:0.75, 1:1.25, 1:1.5 and 1:1.75, were ball milled and then heat treated at 1000 °C for 3 h to complete reactions. The synthesized powders were characterized using x-ray diffraction (XRD), FTIR spectroscopy, scanning electron microscope and high resolution transmission electron microscopy. XRD results showed that the average crystallite size of the powder varies from 53 to 67 nm and crystallinity of powder found to be in the range of 88% and 96%. In vitro biocompatibility studies were carried out using osteoblast (MG63) and fibroblast cells (NIH3T3), demonstrated non-toxic nature of the seashell derived HAp powder.",

keywords = "Biocompatibity, Hydroxyapatite, MTT assay, Mechanochemical synthesis, Sea shell, X-ray diffraction",

author = "Anindya Pal and Shubhadeep Maity and Sumit Chabri and Supriya Bera and Chowdhury, {Amit Roy} and Mitun Das and Arijit Sinha",

note = "Publisher Copyright: {\textcopyright} 2017 IOP Publishing Ltd.",

year = "2017",

month = jan,

day = "13",

doi = "10.1088/2057-1976/aa54f5",

language = "אנגלית",

volume = "3",

journal = "Biomedical Physics and Engineering Express",

issn = "2057-1976",

publisher = "IOP Publishing Ltd.",

number = "1",

}

TY - JOUR

T1 - Mechanochemical synthesis of nanocrystalline hydroxyapatite from Mercenaria clam shells and phosphoric acid

AU - Pal, Anindya

AU - Maity, Shubhadeep

AU - Chabri, Sumit

AU - Bera, Supriya

AU - Chowdhury, Amit Roy

AU - Das, Mitun

AU - Sinha, Arijit

PY - 2017/1/13

Y1 - 2017/1/13

N2 - The aim of the present study is to synthesize hydroxyapatite from seashell and to explore its biocompatibility in vitro. Nano-crystalline hydroxyapatite (HAp) ceramics were successfully fabricated by a mechanochemical method using clam seashells and phosphoric acid. The CaO and H3PO4 acid at different w.t% ratios i.e. 1:0.75, 1:1.25, 1:1.5 and 1:1.75, were ball milled and then heat treated at 1000 °C for 3 h to complete reactions. The synthesized powders were characterized using x-ray diffraction (XRD), FTIR spectroscopy, scanning electron microscope and high resolution transmission electron microscopy. XRD results showed that the average crystallite size of the powder varies from 53 to 67 nm and crystallinity of powder found to be in the range of 88% and 96%. In vitro biocompatibility studies were carried out using osteoblast (MG63) and fibroblast cells (NIH3T3), demonstrated non-toxic nature of the seashell derived HAp powder.

AB - The aim of the present study is to synthesize hydroxyapatite from seashell and to explore its biocompatibility in vitro. Nano-crystalline hydroxyapatite (HAp) ceramics were successfully fabricated by a mechanochemical method using clam seashells and phosphoric acid. The CaO and H3PO4 acid at different w.t% ratios i.e. 1:0.75, 1:1.25, 1:1.5 and 1:1.75, were ball milled and then heat treated at 1000 °C for 3 h to complete reactions. The synthesized powders were characterized using x-ray diffraction (XRD), FTIR spectroscopy, scanning electron microscope and high resolution transmission electron microscopy. XRD results showed that the average crystallite size of the powder varies from 53 to 67 nm and crystallinity of powder found to be in the range of 88% and 96%. In vitro biocompatibility studies were carried out using osteoblast (MG63) and fibroblast cells (NIH3T3), demonstrated non-toxic nature of the seashell derived HAp powder.

KW - Biocompatibity

KW - Hydroxyapatite

KW - MTT assay

KW - Mechanochemical synthesis

KW - Sea shell

KW - X-ray diffraction

UR - http://www.scopus.com/inward/record.url?scp=85123785488&partnerID=8YFLogxK

U2 - 10.1088/2057-1976/aa54f5

DO - 10.1088/2057-1976/aa54f5

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:85123785488

SN - 2057-1976

VL - 3

JO - Biomedical Physics and Engineering Express

JF - Biomedical Physics and Engineering Express

IS - 1

M1 - 015010

ER -

Mechanochemical synthesis of nanocrystalline hydroxyapatite from Mercenaria clam shells and phosphoric acid

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this